CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000002
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alkyldihydroxyacetonephosphate synthase, peroxisomal 
Protein Synonyms/Alias
 Alkyl-DHAP synthase; Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase 
Gene Name
 AGPS 
Gene Synonyms/Alias
 AAG5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
85QESGTIPKKRQEVMKubiquitination[1, 2]
92KKRQEVMKWNGWGYNubiquitination[1, 2]
102GWGYNDSKFIFNKKGacetylation[3]
102GWGYNDSKFIFNKKGubiquitination[1, 2]
169EDFLHDLKETNISYSacetylation[3, 4]
347TPRGIIEKSCQGPRMacetylation[3, 5]
456KFYITKFKGFDPNQLubiquitination[1, 2]
490QVYDIAAKFGGLAAGubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids (By similarity). 
Sequence Annotation
 DOMAIN 202 384 FAD-binding PCMH-type.
 NP_BIND 234 240 FAD (By similarity).
 NP_BIND 303 309 FAD (By similarity).
 NP_BIND 316 319 FAD (By similarity).
 NP_BIND 368 374 FAD (By similarity).
 REGION 615 617 Important for enzyme activity (By
 ACT_SITE 578 578 Proton donor/acceptor (By similarity).
 BINDING 515 515 Substrate (By similarity).
 MOD_RES 65 65 Phosphoserine (By similarity).
 MOD_RES 102 102 N6-acetyllysine.
 MOD_RES 347 347 N6-acetyllysine.  
Keyword
 Acetylation; Cataract; Complete proteome; Disease mutation; Dwarfism; FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Membrane; Peroxisome; Phosphoprotein; Reference proteome; Rhizomelic chondrodysplasia punctata; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 658 AA 
Protein Sequence
MAEAAAAAGG TGLGAGASYG SAADRDRDPD PDRAGRRLRV LSGHLLGRPR EALSTNECKA 60
RRAASAATAA PTATPAAQES GTIPKKRQEV MKWNGWGYND SKFIFNKKGQ IELTGKRYPL 120
SGMGLPTFKE WIQNTLGVNV EHKTTSKASL NPSDTPPSVV NEDFLHDLKE TNISYSQEAD 180
DRVFRAHGHC LHEIFLLREG MFERIPDIVL WPTCHDDVVK IVNLACKYNL CIIPIGGGTS 240
VSYGLMCPAD ETRTIISLDT SQMNRILWVD ENNLTAHVEA GITGQELERQ LKESGYCTGH 300
EPDSLEFSTV GGWVSTRASG MKKNIYGNIE DLVVHIKMVT PRGIIEKSCQ GPRMSTGPDI 360
HHFIMGSEGT LGVITEATIK IRPVPEYQKY GSVAFPNFEQ GVACLREIAK QRCAPASIRL 420
MDNKQFQFGH ALKPQVSSIF TSFLDGLKKF YITKFKGFDP NQLSVATLLF EGDREKVLQH 480
EKQVYDIAAK FGGLAAGEDN GQRGYLLTYV IAYIRDLALE YYVLGESFET SAPWDRVVDL 540
CRNVKERITR ECKEKGVQFA PFSTCRVTQT YDAGACIYFY FAFNYRGISD PLTVFEQTEA 600
AAREEILANG GSLSHHHGVG KLRKQWLKES ISDVGFGMLK SVKEYVDPNN IFGNRNLL 658 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005782; C:peroxisomal matrix; TAS:Reactome.
 GO:0005778; C:peroxisomal membrane; IDA:UniProtKB.
 GO:0008609; F:alkylglycerone-phosphate synthase activity; IDA:UniProtKB.
 GO:0071949; F:FAD binding; ISS:UniProtKB.
 GO:0008762; F:UDP-N-acetylmuramate dehydrogenase activity; IEA:InterPro.
 GO:0008611; P:ether lipid biosynthetic process; ISS:UniProtKB. 
Interpro
 IPR025650; Alkyl-DHAP_Synthase.
 IPR016169; CO_DH_flavot_FAD-bd_sub2.
 IPR016166; FAD-bd_2.
 IPR016167; FAD-bd_2_sub1.
 IPR016164; FAD-linked_Oxase-like_C.
 IPR004113; FAD-linked_oxidase_C.
 IPR006094; Oxid_FAD_bind_N. 
Pfam
 PF02913; FAD-oxidase_C
 PF01565; FAD_binding_4 
SMART
  
PROSITE
 PS51387; FAD_PCMH 
PRINTS