CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016916
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase BRE1B 
Protein Synonyms/Alias
 BRE1-B; RING finger protein 40; Syntaxin-1-interacting RING finger protein; Protein staring 
Gene Name
 Rnf40 
Gene Synonyms/Alias
 Bre1b 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
20GGSGPPEKKLNREEKacetylation[1]
499QNHNHQLKGDAQRYKacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes (By similarity). 
Sequence Annotation
 ZN_FING 949 988 RING-type.  
Keyword
 Chromatin regulator; Coiled coil; Complete proteome; Ligase; Metal-binding; Nucleus; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1002 AA 
Protein Sequence
MSGLSNKRAA GDGGSGPPEK KLNREEKTTT TLIEPIRLGG ISSTEEMDSK VLQFKNKKLA 60
ERLEQRQACE DELRERIEKL EKRQATDDAT LLIVNRYWAQ LDETVEALLQ CYENQRELSS 120
SGTEVPGCQE GLTRDVIPRT DPGTSDLREP LPMQFRAPLS EPALAFVVAL GASSCEEVEL 180
QLQGRMEFSK AAVSRVVEAS DRLQRQVEEL CQRVYSRGDS EAPGEVARAR TRELGRENRR 240
LQDLATQLQE KHHRISLEYS ELQDKVTSTE TKVLEMETTV EDLQWDIEKL RKREQKLNKH 300
LAEALEQLNS GYYVSGSSTG FQGGQITLSM QKFEMLNAEL EENQELANSR MAELEKLQAE 360
LQGAVRTNER LKVALRSLPE EVVRETGEYR MLQAQFSLLY NESLQVKTQL DEARGLLLAS 420
KNSHLRHIEH MESDELGLQK KLRTEVIQLE DTLAQVRKEY EMLRIEFEQN LAANEQAGPI 480
NREMRHLISS LQNHNHQLKG DAQRYKRKLR EVQAEIGKLR AQASGSSHCG PNLSHPDDSG 540
LNALAPGKED SGPGPGGTPD SKKELALVAG ATSVASSVKK EELVSSEDDA QALAPGTQGL 600
PSRGREPEAR PKRELREREG PSLGPPPAAS TLSRADREKA KAEEARRKES ELLKGLRAEL 660
KKAQESQKEM KLLLDMYKSA PKEQRDKVQL MAAERKAKAE VDELRSRIRD LEERDRRESK 720
KIADEDALRR IRQAEEQIEH LQRKLGATKQ EEEALLSEMD VTGQAFEDMQ EQNGRLLQQL 780
REKDDANFKL MSERIKANQI HKLLREEKDE LGEQVLGLKS QVDAQLLTVQ KLEEKERALQ 840
GSLGGVEKEL TLRSQALELN KRKAVEAAQL AEDLKVQLEH VQTRLREIQP CLAESRAARE 900
KESFNLKRAQ EDISRLRRKL EKQRKVEVYA DADEILQEEI KEYKARLTCP CCNTRKKDAV 960
LTKCFHVFCF ECVRGRYEAR QRKCPKCNAA FGAHDFHRVY IS 1002 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0033503; C:HULC complex; ISS:UniProtKB.
 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0032403; F:protein complex binding; IDA:RGD.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:RGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0033523; P:histone H2B ubiquitination; IEA:Compara.
 GO:0010390; P:histone monoubiquitination; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:RGD. 
Interpro
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
  
SMART
 SM00184; RING 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS