CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012360
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pumilio homolog 1 
Protein Synonyms/Alias
 HsPUM; Pumilio-1 
Gene Name
 PUM1 
Gene Synonyms/Alias
 KIAA0099; PUMH1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
107GGGYNNSKHRWPTGDubiquitination[1]
151EQLLPGKKFWETDESubiquitination[1]
160WETDESSKDGPKGIFubiquitination[1]
164ESSKDGPKGIFLGDQubiquitination[1]
270GDKLGDLKEEGDVMDubiquitination[2]
718TGSSDLYKRTSSSLTubiquitination[1, 2, 3]
794AAPGAEAKYRSASSAacetylation[4]
794AAPGAEAKYRSASSAubiquitination[1, 2, 3]
869GSRFIQLKLERATPAubiquitination[1]
914EFGSLEQKLALAERIubiquitination[2]
940YGCRVIQKALEFIPSubiquitination[1, 2]
963ELDGHVLKCVKDQNGubiquitination[1]
976NGNHVVQKCIECVQPubiquitination[1]
1076VLVLSQHKFASNVVEubiquitination[1]
1084FASNVVEKCVTHASRubiquitination[1]
1127YANYVVQKMIDVAEPubiquitination[1, 2]
1163YGKHILAKLEKYYMKubiquitination[1]
1166HILAKLEKYYMKNGVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity). 
Sequence Annotation
 DOMAIN 828 1168 PUM-HD.
 REPEAT 848 883 Pumilio 1.
 REPEAT 884 919 Pumilio 2.
 REPEAT 920 955 Pumilio 3.
 REPEAT 956 991 Pumilio 4.
 REPEAT 992 1027 Pumilio 5.
 REPEAT 1028 1063 Pumilio 6.
 REPEAT 1064 1099 Pumilio 7.
 REPEAT 1103 1142 Pumilio 8.
 MOD_RES 19 19 Phosphoserine.
 MOD_RES 75 75 Phosphoserine.
 MOD_RES 98 98 Phosphoserine.
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 124 124 Phosphoserine (By similarity).
 MOD_RES 209 209 Phosphoserine.
 MOD_RES 709 709 Phosphoserine.
 MOD_RES 806 806 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1186 AA 
Protein Sequence
MSVACVLKRK AVLWQDSFSP HLKHHPQEPA NPNMPVVLTS GTGSQAQPQP AANQALAAGT 60
HSSPVPGSIG VAGRSQDDAM VDYFFQRQHG EQLGGGGSGG GGYNNSKHRW PTGDNIHAEH 120
QVRSMDELNH DFQALALEGR AMGEQLLPGK KFWETDESSK DGPKGIFLGD QWRDSAWGTS 180
DHSVSQPIMV QRRPGQSFHV NSEVNSVLSP RSESGGLGVS MVEYVLSSSP GDSCLRKGGF 240
GPRDADSDEN DKGEKKNKGT FDGDKLGDLK EEGDVMDKTN GLPVQNGIDA DVKDFSRTPG 300
NCQNSANEVD LLGPNQNGSE GLAQLTSTNG AKPVEDFSNM ESQSVPLDPM EHVGMEPLQF 360
DYSGTQVPVD SAAATVGLFD YNSQQQLFQR PNALAVQQLT AAQQQQYALA AAHQPHIGLA 420
PAAFVPNPYI ISAAPPGTDP YTAGLAAAAT LGPAVVPHQY YGVTPWGVYP ASLFQQQAAA 480
AAAATNSANQ QTTPQAQQGQ QQVLRGGASQ RPLTPNQNQQ GQQTDPLVAA AAVNSALAFG 540
QGLAAGMPGY PVLAPAAYYD QTGALVVNAG ARNGLGAPVR LVAPAPVIIS SSAAQAAVAA 600
AAASANGAAG GLAGTTNGPF RPLGTQQPQP QPQQQPNNNL ASSSFYGNNS LNSNSQSSSL 660
FSQGSAQPAN TSLGFGSSSS LGATLGSALG GFGTAVANSN TGSGSRRDSL TGSSDLYKRT 720
SSSLTPIGHS FYNGLSFSSS PGPVGMPLPS QGPGHSQTPP PSLSSHGSSS SLNLGGLTNG 780
SGRYISAAPG AEAKYRSASS ASSLFSPSST LFSSSRLRYG MSDVMPSGRS RLLEDFRNNR 840
YPNLQLREIA GHIMEFSQDQ HGSRFIQLKL ERATPAERQL VFNEILQAAY QLMVDVFGNY 900
VIQKFFEFGS LEQKLALAER IRGHVLSLAL QMYGCRVIQK ALEFIPSDQQ NEMVRELDGH 960
VLKCVKDQNG NHVVQKCIEC VQPQSLQFII DAFKGQVFAL STHPYGCRVI QRILEHCLPD 1020
QTLPILEELH QHTEQLVQDQ YGNYVIQHVL EHGRPEDKSK IVAEIRGNVL VLSQHKFASN 1080
VVEKCVTHAS RTERAVLIDE VCTMNDGPHS ALYTMMKDQY ANYVVQKMID VAEPGQRKIV 1140
MHKIRPHIAT LRKYTYGKHI LAKLEKYYMK NGVDLGPICG PPNGII 1186 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR001313; Pumilio_RNA-bd_rpt. 
Pfam
 PF00806; PUF 
SMART
 SM00025; Pumilio 
PROSITE
 PS50302; PUM
 PS50303; PUM_HD 
PRINTS