| Tag | Content |
|---|
| CPLM ID | CPLM-001791 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Bifunctional aspartokinase/homoserine dehydrogenase 1 |
Protein Synonyms/Alias | Aspartokinase I/homoserine dehydrogenase I; AKI-HDI; Aspartokinase; Homoserine dehydrogenase |
Gene Name | thrA |
Gene Synonyms/Alias | thrA1; thrA2; b0002; JW0001 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 5 | ***MRVLKFGGTSVA | acetylation | [1] | | 42 | TVLSAPAKITNHLVA | acetylation | [1] | | 53 | HLVAMIEKTISGQDA | acetylation | [1] | | 153 | TVIDPVEKLLAVGHY | acetylation | [1] | | 247 | VPDARLLKSMSYQEA | acetylation | [1] | | 263 | ELSYFGAKVLHPRTI | acetylation | [1] | | 283 | FQIPCLIKNTGNPQA | acetylation | [1] | | 383 | EEFYLELKEGLLEPL | acetylation | [1] | | 415 | TLRGISAKFFAALAR | acetylation | [1] | | 529 | QEELAQAKEPFNLGR | acetylation | [1] | | 542 | GRLIRLVKEYHLLNP | acetylation | [1] | | 580 | HVVTPNKKANTSSMD | acetylation | [1] | | 598 | QLRYAAEKSRRKFLY | acetylation | [1] | | 770 | DGNDPLFKVKNGENA | acetylation | [1] | | 817 | LLRTLSWKLGV**** | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | |
Sequence Annotation | DOMAIN 320 394 ACT 1.
DOMAIN 401 478 ACT 2.
NP_BIND 471 478 NADP (Potential).
REGION 1 249 Aspartokinase.
REGION 250 470 Interface.
REGION 471 820 Homoserine dehydrogenase. |
Keyword | Allosteric enzyme; Amino-acid biosynthesis; ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Multifunctional enzyme; NADP; Nucleotide-binding; Oxidoreductase; Reference proteome; Repeat; Threonine biosynthesis; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 820 AA |
Protein Sequence | MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM IEKTISGQDA 60
LPNISDAERI FAELLTGLAA AQPGFPLAQL KTFVDQEFAQ IKHVLHGISL LGQCPDSINA 120
ALICRGEKMS IAIMAGVLEA RGHNVTVIDP VEKLLAVGHY LESTVDIAES TRRIAASRIP 180
ADHMVLMAGF TAGNEKGELV VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRQV 240
PDARLLKSMS YQEAMELSYF GAKVLHPRTI TPIAQFQIPC LIKNTGNPQA PGTLIGASRD 300
EDELPVKGIS NLNNMAMFSV SGPGMKGMVG MAARVFAAMS RARISVVLIT QSSSEYSISF 360
CVPQSDCVRA ERAMQEEFYL ELKEGLLEPL AVTERLAIIS VVGDGMRTLR GISAKFFAAL 420
ARANINIVAI AQGSSERSIS VVVNNDDATT GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL 480
LEQLKRQQSW LKNKHIDLRV CGVANSKALL TNVHGLNLEN WQEELAQAKE PFNLGRLIRL 540
VKEYHLLNPV IVDCTSSQAV ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRYAAEKSR 600
RKFLYDTNVG AGLPVIENLQ NLLNAGDELM KFSGILSGSL SYIFGKLDEG MSFSEATTLA 660
REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELADIE IEPVLPAEFN AEGDVAAFMA 720
NLSQLDDLFA ARVAKARDEG KVLRYVGNID EDGVCRVKIA EVDGNDPLFK VKNGENALAF 780
YSHYYQPLPL VLRGYGAGND VTAAGVFADL LRTLSWKLGV 820 |
Gene Ontology | GO:0016597; F:amino acid binding; IEA:InterPro. GO:0004072; F:aspartate kinase activity; IDA:EcoCyc. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004412; F:homoserine dehydrogenase activity; IDA:EcoCyc. GO:0050661; F:NADP binding; IEA:InterPro. GO:0009090; P:homoserine biosynthetic process; IDA:EcoCyc. GO:0009089; P:lysine biosynthetic process via diaminopimelate; IDA:EcoCyc. GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |