CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002695
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Citrate synthase, peroxisomal 
Protein Synonyms/Alias
  
Gene Name
 CIT2 
Gene Synonyms/Alias
 YCR005C; YCR043; YCR5C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
176ESESKFAKAYAQGISubiquitination[1]
184AYAQGISKQDYWSYTubiquitination[2]
218RNVFKDGKMGEVDPNubiquitination[1, 3]
239LVNLIGSKDEDFVDLubiquitination[1, 2, 3]
354PRYMAQRKFAMDHFPubiquitination[4, 5, 6]
385GVLTEHGKTKNPWPNubiquitination[4, 5, 6]
454EKYKELVKNIESKL*ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [5] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [6] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
  
Sequence Annotation
 MOTIF 458 460 Microbody targeting signal (Potential).
 ACT_SITE 293 293 By similarity.
 ACT_SITE 339 339 By similarity.
 ACT_SITE 394 394 By similarity.
 MOD_RES 21 21 Phosphoserine.
 CROSSLNK 354 354 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 385 385 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Isopeptide bond; Peroxisome; Phosphoprotein; Reference proteome; Transferase; Tricarboxylic acid cycle; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 460 AA 
Protein Sequence
MTVPYLNSNR NVASYLQSNS SQEKTLKERF SEIYPIHAQD VRQFVKEHGK TKISDVLLEQ 60
VYGGMRGIPG SVWEGSVLDP EDGIRFRGRT IADIQKDLPK AKGSSQPLPE ALFWLLLTGE 120
VPTQAQVENL SADLMSRSEL PSHVVQLLDN LPKDLHPMAQ FSIAVTALES ESKFAKAYAQ 180
GISKQDYWSY TFEDSLDLLG KLPVIAAKIY RNVFKDGKMG EVDPNADYAK NLVNLIGSKD 240
EDFVDLMRLY LTIHSDHEGG NVSAHTSHLV GSALSSPYLS LASGLNGLAG PLHGRANQEV 300
LEWLFALKEE VNDDYSKDTI EKYLWDTLNS GRVIPGYGHA VLRKTDPRYM AQRKFAMDHF 360
PDYELFKLVS SIYEVAPGVL TEHGKTKNPW PNVDAHSGVL LQYYGLKESS FYTVLFGVSR 420
AFGILAQLIT DRAIGASIER PKSYSTEKYK ELVKNIESKL 460 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005777; C:peroxisome; IDA:SGD.
 GO:0004108; F:citrate (Si)-synthase activity; TAS:SGD.
 GO:0006101; P:citrate metabolic process; TAS:SGD.
 GO:0006537; P:glutamate biosynthetic process; TAS:SGD.
 GO:0006097; P:glyoxylate cycle; TAS:SGD.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway. 
Interpro
 IPR016142; Citrate_synth-like_lrg_a-sub.
 IPR002020; Citrate_synthase-like.
 IPR016141; Citrate_synthase-like_core.
 IPR019810; Citrate_synthase_AS.
 IPR010109; Citrate_synthase_euk. 
Pfam
 PF00285; Citrate_synt 
SMART
  
PROSITE
 PS00480; CITRATE_SYNTHASE 
PRINTS
 PR00143; CITRTSNTHASE.