| Tag | Content |
|---|
| CPLM ID | CPLM-010229 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Oxygen sensor protein DosP |
Protein Synonyms/Alias | Direct oxygen-sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, |
Gene Name | dosP |
Gene Synonyms/Alias | dos; yddU; b1489; JW1484 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 367 | ALALEQEKSRQHIEQ | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Heme-based oxygen sensor protein displaying phosphodiesterase (PDE) activity toward c-di-GMP in response to oxygen availability. Involved in the modulation of intracellular c-di-GMP levels, in association with DosC which catalyzes the biosynthesis of c-di-GMP (diguanylate cyclase activity). Cyclic- di-GMP is a second messenger which controls cell surface- associated traits in bacteria. Has very poor PDE activity on cAMP (PubMed:15995192) but is not active with cGMP, bis(p-nitrophenyl) phosphate or p-nitrophenyl phosphate (PubMed:11970957). Via its PDE activity on c-di-GMP, DosP regulates biofilm formation through the repression of transcription of the csgBAC operon, which encodes curli structural subunits. |
Sequence Annotation | DOMAIN 10 81 PAS 1.
DOMAIN 134 207 PAS 2.
DOMAIN 208 260 PAC.
DOMAIN 402 532 GGDEF.
DOMAIN 541 795 EAL.
METAL 69 69 Iron (heme proximal ligand).
METAL 87 87 Iron (heme distal ligand). |
Keyword | 3D-structure; c-di-GMP; Complete proteome; Heme; Hydrolase; Iron; Magnesium; Metal-binding; Reference proteome; Repeat; Sensory transduction; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 799 AA |
Protein Sequence | MKLTDADNAA DGIFFPALEQ NMMGAVLINE NDEVMFFNPA AEKLWGYKRE EVIGNNIDML 60
IPRDLRPAHP EYIRHNREGG KARVEGMSRE LQLEKKDGSK IWTRFALSKV SAEGKVYYLA 120
LVRDASVEMA QKEQTRQLII AVDHLDRPVI VLDPERHIVQ CNRAFTEMFG YCISEASGMQ 180
PDTLLNIPEF PADNRIRLQQ LLWKTARDQD EFLLLTRTGE KIWIKASISP VYDVLAHLQN 240
LVMTFSDITE ERQIRQLEGN ILAAMCSSPP FHEMGEIICR NIESVLNESH VSLFALRNGM 300
PIHWASSSHG AEIQNAQSWS ATIRQRDGAP AGILQIKTSS GAETSAFIER VADISQHMAA 360
LALEQEKSRQ HIEQLIQFDP MTGLPNRNNL HNYLDDLVDK AVSPVVYLIG VDHIQDVIDS 420
LGYAWADQAL LEVVNRFREK LKPDQYLCRI EGTQFVLVSL ENDVSNITQI ADELRNVVSK 480
PIMIDDKPFP LTLSIGISYD LGKNRDYLLS TAHNAMDYIR KNGGNGWQFF SPAMNEMVKE 540
RLVLGAALKE AISNNQLKLV YQPQIFAETG ELYGIEALAR WHDPLHGHVP PSRFIPLAEE 600
IGEIENIGRW VIAEACRQLA EWRSQNIHIP ALSVNLSALH FRSNQLPNQV SDAMHAWGID 660
GHQLTVEITE SMMMEHDTEI FKRIQILRDM GVGLSVDDFG TGFSGLSRLV SLPVTEIKID 720
KSFVDRCLTE KRILALLEAI TSIGQSLNLT VVAEGVETKE QFEMLRKIHC RVIQGYFFSR 780
PLPAEEIPGW MSSVLPLKI 799 |
Gene Ontology | GO:0071111; F:cyclic-guanylate-specific phosphodiesterase activity; IDA:EcoCyc. GO:0020037; F:heme binding; IDA:EcoCyc. GO:0000287; F:magnesium ion binding; IMP:EcoCyc. GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro. GO:0016849; F:phosphorus-oxygen lyase activity; IEA:InterPro. GO:0009190; P:cyclic nucleotide biosynthetic process; IEA:InterPro. GO:0035556; P:intracellular signal transduction; IEA:InterPro. GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW. GO:0023014; P:signal transduction by phosphorylation; IEA:GOC. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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