CPLM-021539

Genbank Nucleotide ID

CCR4-NOT transcription complex subunit 10

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
74	TAVAEFFKSNQTTTD	ubiquitination	[1]
177	HLLAVLEKMISQGNN	acetylation	[2]
204	NKDGSNHKAESGALI	ubiquitination	[1, 3]
256	SAPSLFLKSNFEYLR	ubiquitination	[1, 4]
271	GNYRKAVKLLNSSNI	ubiquitination	[5]
404	ECCIAANKGTSEQET	ubiquitination	[4]
713	NQLLPAVKTHSEVRK	ubiquitination	[3]
720	KTHSEVRKKPVFQPV	ubiquitination	[6]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]

Functional Description

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4- NOT complex.

MOD_RES 2 2 N-acetylalanine.

Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Nucleus; Polymorphism; Reference proteome; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR001440; TPR-1.
IPR011990; TPR-like_helical.
IPR019734; TPR_repeat.