CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023521
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor-like protein 3 
Protein Synonyms/Alias
  
Gene Name
 Arl3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
35AGKTTLLKQLASEDIacetylation[1]
54PTQGFNIKSVQSQGFacetylation[1]
54PTQGFNIKSVQSQGFubiquitination[2]
62SVQSQGFKLNVWDIGubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (By similarity). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. 
Sequence Annotation
 NP_BIND 24 31 GTP.
 NP_BIND 67 71 GTP (By similarity).
 NP_BIND 126 129 GTP.
 NP_BIND 159 161 GTP.
 METAL 31 31 Magnesium.
 METAL 48 48 Magnesium.
 BINDING 48 48 GTP.
 BINDING 70 70 GTP; via amide nitrogen.
 MOD_RES 5 5 Phosphoserine (By similarity).
 LIPID 2 2 N-myristoyl glycine (Potential).  
Keyword
 3D-structure; Cell cycle; Cell division; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Golgi apparatus; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 182 AA 
Protein Sequence
MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG FNIKSVQSQG 60
FKLNVWDIGG QRKIRPYWRS YFENTDILIY VIDSADRKRF EETGQELTEL LEEEKLSCVP 120
VLIFANKQDL LTAAPASEIA EGLNLHTIRD RVWQIQSCSA LTGEGVQDGM NWVCKNVNAK 180
KK 182 
Gene Ontology
 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0005881; C:cytoplasmic microtubule; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0032391; C:photoreceptor connecting cilium; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005876; C:spindle microtubule; ISS:UniProtKB.
 GO:0019003; F:GDP binding; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IDA:UniProtKB.
 GO:0003924; F:GTPase activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008017; F:microtubule binding; ISS:UniProtKB.
 GO:0060271; P:cilium morphogenesis; ISS:UniProtKB.
 GO:0000910; P:cytokinesis; ISS:UniProtKB.
 GO:0042073; P:intraflagellar transport; IMP:MGI.
 GO:0001822; P:kidney development; IMP:UniProtKB.
 GO:0042461; P:photoreceptor cell development; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0007264; P:small GTPase mediated signal transduction; ISS:UniProtKB. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR024156; Small_GTPase_ARF.
 IPR006689; Small_GTPase_ARF/SAR. 
Pfam
 PF00025; Arf 
SMART
 SM00177; ARF 
PROSITE
 PS51417; ARF 
PRINTS
 PR00328; SAR1GTPBP.