UniProt Accession

 EHD2_MOUSE; Q8BH64; Q8BL28 

Genbank Protein ID

 AY531389; AK031203; AK046566; AK048356; AK050243; AK084455; AK085016; BC113161 

Genbank Nucleotide ID

 AAS48536.1; BAC27298.1; BAC32789.1; BAC33310.1; BAC34142.1; BAC39188.1; BAC39339.1; AAI13162.1 

Protein Name

 EH domain-containing protein 2 

Protein Synonyms/Alias

  

Gene Name

 Ehd2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
6	**MFSWLKKGGARGQ	ubiquitination	[1]
58	EDADFDGKPMVLVAG	ubiquitination	[1]
124	DPEKPFRKLNPFGNT	ubiquitination	[1]
452	VVTKDKSKYDEIFYN	acetylation	[2]
452	VVTKDKSKYDEIFYN	ubiquitination	[1]
466	NLAPADGKLSGSKAK	ubiquitination	[1]
473	KLSGSKAKTWMVGTK	ubiquitination	[1]
480	KTWMVGTKLPNSVLG	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane. Binds ATP; does not bind GTP. 

Sequence Annotation

 DOMAIN 449 537 EH.
 DOMAIN 481 516 EF-hand.
 NP_BIND 65 72 ATP.
 BINDING 220 220 ATP.
 BINDING 258 258 ATP.
 MOD_RES 438 438 Phosphoserine.
 MOD_RES 468 468 Phosphoserine.  

Keyword

 3D-structure; ATP-binding; Calcium; Cell membrane; Complete proteome; Endosome; Hydrolase; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 543 AA 

Protein Sequence

Gene Ontology

 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0055038; C:recycling endosome membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0019904; F:protein domain specific binding; IDA:MGI.
 GO:0030866; P:cortical actin cytoskeleton organization; IDA:MGI.
 GO:0032456; P:endocytic recycling; ISS:UniProtKB.
 GO:0006897; P:endocytosis; IDA:MGI.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:2001137; P:positive regulation of endocytic recycling; IMP:UniProtKB.
 GO:1901741; P:positive regulation of myoblast fusion; IMP:UniProtKB.
 GO:0072659; P:protein localization to plasma membrane; IDA:UniProtKB. 

Interpro

 IPR001401; Dynamin_GTPase.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00350; Dynamin_N 

SMART

 SM00027; EH 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH 

PRINTS