CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-027396
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Isoleucine-tRNA ligase, putative 
Protein Synonyms/Alias
  
Gene Name
 PF13_0179 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Plasmodium falciparum (isolate 3D7) 
NCBI Taxa ID
 36329 
Lysine Modification
Position
Peptide
Type
References
328VLYDCYMKSEKCDTSacetylation[1]
450KWNVENIKIVNRFLGacetylation[1]
464GSELKELKYKPLFTNacetylation[1]
800DPLYILNKYGADSLRacetylation[1]
1058KSLTILHKNESFFKHacetylation[1]
1064HKNESFFKHFDRISNacetylation[1]
1230LNENEYIKKCLRRNLacetylation[1]
Reference
 [1] Extensive lysine acetylation occurs in evolutionarily conserved metabolic pathways and parasite-specific functions during Plasmodium falciparum intraerythrocytic development.
 Miao J, Lawrence M, Jeffers V, Zhao F, Parker D, Ge Y, Sullivan WJ Jr, Cui L.
 Mol Microbiol. 2013 Aug;89(4):660-75. [PMID: 23796209] 
Functional Description
  
Sequence Annotation
  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1272 AA 
Protein Sequence
MLRFVNESFL KVSNKELVHI NIHISRNNLF FNKRIIRRVD NIIRVDIDRL SYYISGYRVN 60
KLLSFVNFNK RIHTLRLEEM VTLTFTGISD NPNIVEEEER ILKYWEDIDA FNLSNELSKN 120
KKPYIFYDGP PFATGLPHYG HLLAGIIKDC VTRYFYQSNF YVERRFGWDC HGLPVEYEIE 180
KENNINKRED ILNMGIDVYN EKCRGIVLKY SKEWVATVQR IGRWIDFKND YKTMDKSFME 240
SVWWVFSELY KNNYIYKSFK VMPYSCKCNT PISNFELNLN YKDTPDPSII ISFILCSDFP 300
KVKNVYNIQD DIQPLVQKYS VLYDCYMKSE KCDTSNEKHA DEDIYSDHIN VNKNNNKNNN 360
SNNNNNNNTN CNDNNNINLN YNNNCYAQHC EVFAWTTTPW TLPSNLALCV NEHFIYLRVY 420
HIESQRMFIF AECRLEWIMK ELKWNVENIK IVNRFLGSEL KELKYKPLFT NFYEKHNFKE 480
RAYKIIADDF VSDDAGTGIV HCAPSYGEDD YRVCCKYGII DPESNILIDP LDWNGYFTNE 540
VEELKDMYIK DADNVIKKKL KENNRLMSNN TIVHSYPFCW RSDTPLIYRA IPAWFVRVSN 600
STNKLVENNE TTYWIPAHVK EKKFHNWIKD AKDWCISRNR YWGTPIPIWC DEKMETVICV 660
ESIKHLEELS GIKNINDLHR HYIDNIEIKN PKGDTYPKLK RIPEVFDCWF ESGSMPYAKV 720
HYPFSTETND FHKIFPADFI AEGLDQTRGW FYTLLVISTL LFNKAPFKNL ICNGLVLASD 780
GKKMSKRLKN YPDPLYILNK YGADSLRLYL INSVAVRAEN LKFQEKGVNE IVKSFILPYY 840
HSFRFFSQEV TRYETTNKMQ FLFNTDYIYK NDNIMDQWIF SSLQSLINSV HTEMKAYKLY 900
NVLPKLLNFI ENLTNWYIRL NRDRMRGMLG KENTHQSLNV LCRTLYLFTI IMAPFTPFIS 960
EYIYQFLKNI KYTNNQNMEH NENNETTNIK EEDLNRNDIH KSVHFIMLPQ VDDKYIIKYE 1020
FIELIEHMKD VILLGRNLRE KRKTPNKKPL KSLTILHKNE SFFKHFDRIS NYIKEELNIL 1080
NVEYSNDISC LNFSAIPNFK KLGVKLGYNI KNIQNKIKNM NAEDIKLFQQ NKQIIIDNIL 1140
LEQDDIIIQM NHNIQNDNTE AISNNYITIL MDFTADQQLE NMASAREICN HIQKIRKNLS 1200
LNQNSPVLMH IYIYDQTFKN HMLNENEYIK KCLRRNLNII DLEKDVQNLT DKFFEEKITV 1260
NEKEVLVIFT NQ 1272 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004822; F:isoleucine-tRNA ligase activity; IEA:EC.
 GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002300; aa-tRNA-synth_Ia.
 IPR002301; Ile-tRNA-ligase.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd.
 IPR013155; V/L/I-tRNA-synth_anticodon-bd.
 IPR009008; Val/Leu/Ile-tRNA-synth_edit. 
Pfam
 PF08264; Anticodon_1
 PF00133; tRNA-synt_1 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00984; TRNASYNTHILE.