UniProt Accession

 NDUS1_RAT; Q66HF1 

Genbank Protein ID

 BC081892 

Genbank Nucleotide ID

 AAH81892.1 

Protein Name

 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 Ndufs1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
84	MCLVEIEKAPKVVAA	acetylation	[1]
163	GKRAVEDKNIGPLVK	acetylation	[1]
170	KNIGPLVKTIMTRCI	acetylation	[1]
298	RFAYDGLKRQRLTEP	acetylation	[1]
311	EPMVRNEKGLLTYTS	acetylation	[1]
450	DHLGDSPKILQDIAS	acetylation	[1]
464	SGNHEFSKVLNAAKK	acetylation	[1]
499	AVSSIAQKIRVASGA	acetylation	[1]
511	SGAAAEWKVMNILHR	acetylation	[1]
543	AIRKNPPKLLFLLGA	acetylation	[1]
562	ITRQDLPKDCFIVYQ	acetylation	[1]
621	GLAREDWKIIRALSE	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity). 

Sequence Annotation

 DOMAIN 30 108 2Fe-2S ferredoxin-type.
 DOMAIN 245 301 4Fe-4S Mo/W bis-MGD-type.
 METAL 64 64 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 75 75 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 78 78 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 92 92 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 124 124 Iron-sulfur 2 (4Fe-4S); via pros nitrogen
 METAL 128 128 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 131 131 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 137 137 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 176 176 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 179 179 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 182 182 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 226 226 Iron-sulfur 3 (4Fe-4S) (By similarity).
 MOD_RES 84 84 N6-acetyllysine (By similarity).  

Keyword

 2Fe-2S; 4Fe-4S; Acetylation; Complete proteome; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Reference proteome; Respiratory chain; Transit peptide; Transport; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 727 AA 

Protein Sequence

Gene Ontology

 GO:0005758; C:mitochondrial intermembrane space; ISS:UniProtKB.
 GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
 GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IBA:RefGenome.
 GO:0008637; P:apoptotic mitochondrial changes; ISS:UniProtKB.
 GO:0046034; P:ATP metabolic process; ISS:UniProtKB.
 GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
 GO:0045333; P:cellular respiration; ISS:UniProtKB.
 GO:0072593; P:reactive oxygen species metabolic process; ISS:UniProtKB.
 GO:0051881; P:regulation of mitochondrial membrane potential; ISS:UniProtKB. 

Interpro

 IPR001041; 2Fe-2S_ferredoxin-type.
 IPR012675; Beta-grasp_dom.
 IPR006656; Mopterin_OxRdtase.
 IPR000283; NADH_UbQ_OxRdtase_75kDa_su_CS.
 IPR010228; NADH_UbQ_OxRdtase_Gsu.
 IPR019574; NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
 IPR015405; NuoG_C. 

Pfam

 PF09326; DUF1982
 PF00384; Molybdopterin
 PF10588; NADH-G_4Fe-4S_3 

SMART

 SM00929; NADH-G_4Fe-4S_3 

PROSITE

 PS51085; 2FE2S_FER_2
 PS51669; 4FE4S_MOW_BIS_MGD
 PS00641; COMPLEX1_75K_1
 PS00642; COMPLEX1_75K_2
 PS00643; COMPLEX1_75K_3 

PRINTS