CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005427
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Suppressor of yeast profilin deletion 
Protein Synonyms/Alias
  
Gene Name
 SYP1 
Gene Synonyms/Alias
 YCR030C; YCR30C/YCR29C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
113EELKSDLKSSTEYYNubiquitination[1]
200YNRLDTLKNCMLRFQubiquitination[1]
242SEIDRFAKDASQYNFubiquitination[1]
256FQLSSSSKEVVPNNAubiquitination[1, 2]
394ATSSIYQKQRRPTYSubiquitination[1]
404RPTYSSSKSNNWTPGubiquitination[1]
470PDSSNLPKTVPISISubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Multi-functional protein that contributes to the endocytic process, but also to events that occur at the neck during budding and/or cytokinesis. Plays a role as an endocytic adapters with membrane-tubulation activity that associates with transmembrane cargo proteins and initiates the formation of endocytic sites. Contributes to the stabilization of the nascent clathrin-coated pit. Plays also a role in late endocytosis by mediating vesiculation. Involved in the regulation of cell cycle- dependent dynamics of the septin cytoskeleton by promoting septin turnover in different cell cycle stages. May act through the RHO2 signaling pathway to repolarize cortical actin patches in profilin-deficient cells. 
Sequence Annotation
 DOMAIN 609 869 MHD.
 MOD_RES 264 264 Phosphoserine.
 MOD_RES 331 331 Phosphoserine.
 MOD_RES 416 416 Phosphothreonine.
 MOD_RES 496 496 Phosphoserine.
 MOD_RES 500 500 Phosphoserine.
 MOD_RES 577 577 Phosphothreonine.  
Keyword
 3D-structure; Cell cycle; Complete proteome; Endocytosis; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 870 AA 
Protein Sequence
MTEQRTKYAD SILTTKSPYE ATETIRIRLS QVKLLNKDFY LLFKELANLK RNYAQQLRKI 60
IAENEDITKI LNAQMIESNV LTPQEMSAFR FNSLGELRNV WDTVIEELKS DLKSSTEYYN 120
TLDQQVVREL KESVENNTSW RESKDLHSKL SKNAASIEHY SKNNENSSHL EEARRQWDQQ 180
SPYLFELFET IDYNRLDTLK NCMLRFQTSF SDYLLNTTKE CETVMTKFLA FEPQSEIDRF 240
AKDASQYNFQ LSSSSKEVVP NNASPASATG ARPVSVSNGA ANTEREKKSP QKDKRKSAFG 300
NIGHRLASAS SSLTHNDLMN NEFSDSTNNS SLKSKKSSHT LRSKVGSIFG RNKTKNKRQQ 360
QSSSNSHIQA SITETPNNSS TRVSSTATSS IYQKQRRPTY SSSKSNNWTP GEASDTPPLP 420
PHATPKNVDA PVTADTPPAQ TFTPSEVPPS TPQQSSPPTA KEPDSSNLPK TVPISISQPP 480
LQPQSKTKPL PVEPASPSIS LPTATVDNQP SGQVDSRPLH IRAPALPPSR KQNFIHNRDS 540
QLYDSLPNHG SGATPTSSSL SSIPQERPVS TLSSQITGEL RELNPQATGS STSLVGQSLF 600
QHSSLDTSQF GLNASIAEVL NASFKDGMLQ NSQLIGEIAL NYLPNSVMNS PLPIGINLRI 660
NNGAKFEKVI LNQAFIERVA PEEFKVNPSF IDSRTLGAIK YSIKEPIAPI VIHPVWRFES 720
HQASVVLTVK MSPSLPDEIS QIVIEDLVVF VNIDGANATS ALSKPQGSFS KEKKRITWRF 780
KEPVVLTRNG EGQRLIARFI TDGLAHESAK GVITKFTISE TDNVALPHSG AGSGITLTCQ 840
ELDENNPFGG EWLDVNTKRT LTTGNYHGLA 870 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:UniProtKB.
 GO:0005934; C:cellular bud tip; IDA:UniProtKB.
 GO:0001400; C:mating projection base; IDA:UniProtKB.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0000147; P:actin cortical patch assembly; IMP:SGD.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0032185; P:septin cytoskeleton organization; IMP:SGD. 
Interpro
 IPR018808; Muniscin_C-term_mu_dom. 
Pfam
 PF10291; muHD 
SMART
  
PROSITE
 PS51072; MHD 
PRINTS