CPLM-022179

Genbank Nucleotide ID

Protection of telomeres protein 1

Protein Synonyms/Alias

hPot1; POT1-like telomere end-binding protein

Homo sapiens (Human)

Position	Peptide	Type	References
469	EICKLSNKFNSVIPV	ubiquitination	[1, 2, 3]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.

REGION 33 48 DNA binding.
REGION 270 273 DNA binding.

3D-structure; Alternative splicing; Chromosome; Complete proteome; DNA-binding; Nucleus; Polymorphism; Reference proteome; Telomere.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000783; C:nuclear telomere cap complex; IDA:BHF-UCL.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0043047; F:single-stranded telomeric DNA binding; IMP:UniProtKB.
GO:0010521; F:telomerase inhibitor activity; IDA:BHF-UCL.
GO:0032508; P:DNA duplex unwinding; IDA:BHF-UCL.
GO:0032211; P:negative regulation of telomere maintenance via telomerase; IMP:BHF-UCL.
GO:0060383; P:positive regulation of DNA strand elongation; IDA:BHF-UCL.
GO:0051096; P:positive regulation of helicase activity; IDA:BHF-UCL.
GO:0051973; P:positive regulation of telomerase activity; IDA:BHF-UCL.
GO:0032212; P:positive regulation of telomere maintenance via telomerase; IMP:BHF-UCL.
GO:0016233; P:telomere capping; IMP:BHF-UCL.
GO:0032203; P:telomere formation via telomerase; IDA:BHF-UCL.
GO:0007004; P:telomere maintenance via telomerase; IDA:UniProtKB.

IPR012340; NA-bd_OB-fold.
IPR011564; Telomer_end-bd.