CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009907
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PTS system glucose-specific EIICB component 
Protein Synonyms/Alias
 EIICB-Glc; EII-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component 
Gene Name
 ptsG 
Gene Synonyms/Alias
 glcA; umg; b1101; JW1087 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
11NAFANLQKVGKSLMLacetylation[1]
139FNRFYRIKLPEYLGFacetylation[1]
150YLGFFAGKRFVPIISacetylation[1]
273IAIWHSAKPENRAKVacetylation[1]
382LIKALDLKTPGREDAacetylation[1]
394EDATEDAKATGTSEMacetylation[1]
434VSVADVSKVDQAGLKacetylation[1]
441KVDQAGLKKLGAAGVacetylation[1]
467GTKSDNLKTEMDEYIacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport. This enzyme is also a chemoreceptor monitoring the environment for changes in sugar concentration. 
Sequence Annotation
 DOMAIN 1 388 PTS EIIC type-1.
 DOMAIN 399 477 PTS EIIB type-1.
 ACT_SITE 421 421 Phosphocysteine intermediate; for EIIB  
Keyword
 3D-structure; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Kinase; Membrane; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 477 AA 
Protein Sequence
MFKNAFANLQ KVGKSLMLPV SVLPIAGILL GVGSANFSWL PAVVSHVMAE AGGSVFANMP 60
LIFAIGVALG FTNNDGVSAL AAVVAYGIMV KTMAVVAPLV LHLPAEEIAS KHLADTGVLG 120
GIISGAIAAY MFNRFYRIKL PEYLGFFAGK RFVPIISGLA AIFTGVVLSF IWPPIGSAIQ 180
TFSQWAAYQN PVVAFGIYGF IERCLVPFGL HHIWNVPFQM QIGEYTNAAG QVFHGDIPRY 240
MAGDPTAGKL SGGFLFKMYG LPAAAIAIWH SAKPENRAKV GGIMISAALT SFLTGITEPI 300
EFSFMFVAPI LYIIHAILAG LAFPICILLG MRDGTSFSHG LIDFIVLSGN SSKLWLFPIV 360
GIGYAIVYYT IFRVLIKALD LKTPGREDAT EDAKATGTSE MAPALVAAFG GKENITNLDA 420
CITRLRVSVA DVSKVDQAGL KKLGAAGVVV AGSGVQAIFG TKSDNLKTEM DEYIRNH 477 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005355; F:glucose transmembrane transporter activity; IEA:InterPro.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:EC.
 GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro.
 GO:0016052; P:carbohydrate catabolic process; IMP:EcoCyc.
 GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW. 
Interpro
 IPR018113; PTrfase_EIIB/Cys_phosph_CS.
 IPR004719; PTrfase_sys_maltose/Glc-sp_IIC.
 IPR001996; PTS_EIIB_1.
 IPR003352; PTS_EIIC.
 IPR013013; PTS_EIIC_1.
 IPR011535; PTS_Glc-like_IIB_component.
 IPR011299; PTS_IIBC_glc. 
Pfam
 PF00367; PTS_EIIB
 PF02378; PTS_EIIC 
SMART
  
PROSITE
 PS51098; PTS_EIIB_TYPE_1
 PS01035; PTS_EIIB_TYPE_1_CYS
 PS51103; PTS_EIIC_TYPE_1 
PRINTS