CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029846
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 TRAF family member-associated NF-kappa-B activator 
Protein Synonyms/Alias
  
Gene Name
 TRAF2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21LLQPGFSKTLLGTKLubiquitination[1]
27SKTLLGTKLEAKYLCubiquitination[2, 3, 4, 5, 6, 7]
31LGTKLEAKYLCSACRubiquitination[5]
119CTWKGTLKEYESCHEubiquitination[4, 5]
140LTECPACKGLVRLGEubiquitination[1, 3, 5, 8]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 172 AA 
Protein Sequence
MAAASVTPPG SLELLQPGFS KTLLGTKLEA KYLCSACRNV LRRPFQAQCG HRYCSFCLAS 60
ILSSGPQNCA ACVHEGIYEE GISILESSSA FPDNAARREV ESLPAVCPSD GCTWKGTLKE 120
YESCHEGRCP LMLTECPACK GLVRLGEKER HLEHECPERS LSCRHCRAPC CG 172 
Gene Ontology
 GO:0035631; C:CD40 receptor complex; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0009898; C:internal side of plasma membrane; IEA:Compara.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007250; P:activation of NF-kappaB-inducing kinase activity; IEA:InterPro.
 GO:0043623; P:cellular protein complex assembly; IEA:Compara.
 GO:0051865; P:protein autoubiquitination; IEA:InterPro.
 GO:0030163; P:protein catabolic process; IEA:Compara.
 GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
 GO:0051023; P:regulation of immunoglobulin secretion; IEA:Compara.
 GO:0046328; P:regulation of JNK cascade; IEA:Compara.
 GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IEA:InterPro. 
Interpro
 IPR027133; TRAF2.
 IPR018957; Znf_C3HC4_RING-type.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS.
 IPR001293; Znf_TRAF. 
Pfam
 PF00097; zf-C3HC4 
SMART
 SM00184; RING 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2
 PS50145; ZF_TRAF 
PRINTS