CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004308
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic peptide chain release factor GTP-binding subunit ERF3A 
Protein Synonyms/Alias
 Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog 
Gene Name
 GSPT1 
Gene Synonyms/Alias
 ERF3A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
74RQLNVNAKPFVPNVHubiquitination[1]
289AYFETEKKHFTILDAubiquitination[2]
377RYEECKEKLVPFLKKubiquitination[2]
457MGTVVLGKLESGSICubiquitination[2]
504ENLKIRLKGIEEEEIubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. 
Sequence Annotation
 NP_BIND 81 88 GTP (By similarity).
 NP_BIND 158 162 GTP (By similarity).
 NP_BIND 220 223 GTP (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; GTP-binding; Nonsense-mediated mRNA decay; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 499 AA 
Protein Sequence
MDPGSGGGGG GGGGGGSSSG SSSSDSAPDC WDQADMEAPG PGPCGGGGSL AAAAEAQREN 60
LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAAPPPPV GGAANNHGAG SGAGGRAAPV 120
ESSQEEQSLC EGSNSAVSME LSEPIENGET EMSPEESWEH KEEISEAEPG GGSLGDGRPP 180
EESAHEMMEE EEEIPKPKSV VAPPGAPKKE HVNVVFIGHV DAGKSTIGGQ IMYLTGMVDK 240
RTLEKYEREA KEKNRETWYL SWALDTNQEE RDKGKTVEVG RAYFETEKKH FTILDAPGHK 300
SFVPNMIGGA SQADLAVLVI SARKGEFETG FEKGGQTREH AMLAKTAGVK HLIVLINKMD 360
DPTVNWSNER YEECKEKLVP FLKKVGFNPK KDIHFMPCSG LTGANLKEQS DFCPWYIGLP 420
FIPYLDNLPN FNRSVDGPIR LPIVDKYKDM GTVVLGKLES GSICKGQQLV MMPNKHNVEV 480
LGILSDDVET DTVAPGENLK IRLKGIEEEE ILPGFILCDP NNLCHSGRTF DAQIVIIEHK 540
SIICPGYNAV LHIHTCIEEV EITALICLVD KKSGEKSKTR PRFVKQDQVC IARLRTAGTI 600
CLETFKDFPQ MGRFTLRDEG KTIAIGKVLK LVPEKD 636 
Gene Ontology
 GO:0005622; C:intracellular; NAS:UniProtKB.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; TAS:UniProtKB.
 GO:0003747; F:translation release factor activity; IMP:UniProtKB.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:UniProtKB.
 GO:0006479; P:protein methylation; IDA:MGI. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR004160; Transl_elong_EFTu/EF1A_C.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF03143; GTP_EFTU_D3 
SMART
  
PROSITE
 PS00301; EFACTOR_GTP 
PRINTS
 PR00315; ELONGATNFCT.