UniProt Accession

 NPL4_HUMAN; Q8TAT6; Q8N3J1; Q9H8V2; Q9H964; Q9NWR5; Q9P229 

Genbank Protein ID

 AB040932; AK000664; AK023046; AK023272; BC025930; AL834300 

Genbank Nucleotide ID

 BAA96023.1; BAA91314.1; BAB14372.1; BAB14499.1; AAH25930.1; CAD38971.1 

Protein Name

 Nuclear protein localization protein 4 homolog 

Protein Synonyms/Alias

 Protein NPL4 

Gene Name

 NPLOC4 

Gene Synonyms/Alias

 KIAA1499; NPL4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
62	EITASSNKSLNLLKI	ubiquitination	[1]
68	NKSLNLLKIKHGDLL	ubiquitination	[2]
116	EIDQYLSKQDGKIYR	ubiquitination	[1]
137	CRHGPLGKCVHCVPL	ubiquitination	[3]
243	RFLDFWRKTGNQHFG	ubiquitination	[2]
260	YGRYTEHKDIPLGIR	ubiquitination	[1, 2]
302	VVDEIAAKLGLRKVG	ubiquitination	[1, 2, 4]
307	AAKLGLRKVGWIFTD	ubiquitination	[1, 2]
413	APELGYAKESSSEQY	ubiquitination	[1]
427	YVPDVFYKDVDKFGN	ubiquitination	[1]
431	VFYKDVDKFGNEITQ	ubiquitination	[2]
544	EELAQTWKRSEQWAT	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865] 

Functional Description

 The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). 

Sequence Annotation

 ZN_FING 580 608 RanBP2-type.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Alternative splicing; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; Metal-binding; Nucleus; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 608 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005783; C:endoplasmic reticulum; ISS:HGNC.
 GO:0042175; C:nuclear outer membrane-endoplasmic reticulum membrane network; ISS:HGNC.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006944; P:cellular membrane fusion; ISS:HGNC.
 GO:0030433; P:ER-associated protein catabolic process; ISS:HGNC.
 GO:0007030; P:Golgi organization; ISS:HGNC. 

Interpro

 IPR007717; NPL4.
 IPR024682; Npl4_Ub-like_dom.
 IPR007716; NPL4_Zn-bd_put.
 IPR016563; PolyUb_recognition_cplx_Npl4.
 IPR001876; Znf_RanBP2. 

Pfam

 PF05021; NPL4
 PF11543; UN_NPL4
 PF05020; zf-NPL4 

SMART

 SM00547; ZnF_RBZ 

PROSITE

 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2 

PRINTS