CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008942
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bax inhibitor 1 
Protein Synonyms/Alias
 BI-1; Testis-enhanced gene transcript protein; Transmembrane BAX inhibitor motif-containing protein 6 
Gene Name
 TMBIM6 
Gene Synonyms/Alias
 BI1; TEGT 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MNIFDRKINFDALLubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
15INFDALLKFSHITPSubiquitination[4, 7]
28PSTQQHLKKVYASFAubiquitination[4, 7]
230MILAMNEKDKKKEKKubiquitination[4]
Reference
 [1] Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry.
 Meierhofer D, Wang X, Huang L, Kaiser P.
 J Proteome Res. 2008 Oct;7(10):4566-76. [PMID: 18781797]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Suppressor of apoptosis. Modulates unfolded protein response signaling. Modulate ER calcium homeostasis by acting as a calcium-leak channel. 
Sequence Annotation
 CROSSLNK 7 7 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Apoptosis; Calcium; Complete proteome; Endoplasmic reticulum; Isopeptide bond; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 237 AA 
Protein Sequence
MNIFDRKINF DALLKFSHIT PSTQQHLKKV YASFALCMFV AAAGAYVHMV THFIQAGLLS 60
ALGSLILMIW LMATPHSHET EQKRLGLLAG FAFLTGVGLG PALEFCIAVN PSILPTAFMG 120
TAMIFTCFTL SALYARRRSY LFLGGILMSA LSLLLLSSLG NVFFGSIWLF QANLYVGLVV 180
MCGFVLFDTQ LIIEKAEHGD QDYIWHCIDL FLDFITVFRK LMMILAMNEK DKKKEKK 237 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IEA:InterPro.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW. 
Interpro
 IPR006213; Bax_inhbtr1_CS.
 IPR006214; Bax_inhibitor_1-related. 
Pfam
 PF01027; Bax1-I 
SMART
  
PROSITE
 PS01243; BI1 
PRINTS