UniProt Accession

 HNRPC_HUMAN; P07910; D3DS19; D3DS22; P22628; Q53EX2; Q59FD3; Q5FWE8; Q86SF8; Q86U45; Q96HK7; Q96HM4; Q96IY5; Q9BTS3 

Genbank Protein ID

 M29063; M16342; BX161480; BX247961; BX247992; AB209527; AK223517; CH471078; CH471078; CH471078; CH471078; CH471078; CH471078; CH471078; BC003394; BC007052; BC008364; BC008423; BC066932; BC089438; BC108658; BC103758 

Genbank Nucleotide ID

 AAA36576.1; AAA52680.1; CAD61934.1; CAD62300.1; CAD62326.1; BAD92764.1; BAD97237.1; EAW66392.1; EAW66393.1; EAW66394.1; EAW66395.1; EAW66396.1; EAW66399.1; EAW66400.1; AAH03394.1; AAH07052.1; AAH08364.1; AAH08423.1; AAH66932.1; AAH89438.1; AAI08659.1; AAI03759.1 

Protein Name

 Heterogeneous nuclear ribonucleoproteins C1/C2 

Protein Synonyms/Alias

 hnRNP C1/C2 

Gene Name

 HNRNPC 

Gene Synonyms/Alias

 HNRPC 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
8	MASNVTNKTDPRSMN	ubiquitination	[1]
29	NLNTLVVKKSDVEAI	ubiquitination	[1, 2, 3]
39	DVEAIFSKYGKIVGC	ubiquitination	[1, 2, 3]
42	AIFSKYGKIVGCSVH	ubiquitination	[1]
89	INLAAEPKVNRGKAG	ubiquitination	[1, 3]
163	GKSGFNSKSGQRGSS	ubiquitination	[2]
176	SSKSGKLKGDDLQAI	ubiquitination	[1]
185	DDLQAIKKELTQIKQ	ubiquitination	[1, 3]
193	ELTQIKQKVDSLLEN	ubiquitination	[1, 3]
219	AVEMKNDKSEEEQSS	ubiquitination	[2]
230	EQSSSSVKKDETNVK	ubiquitination	[2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. 

Sequence Annotation

 DOMAIN 16 87 RRM.
 MOTIF 155 161 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 109 109 Phosphothreonine.
 MOD_RES 113 113 Phosphoserine.
 MOD_RES 115 115 Phosphoserine.
 MOD_RES 162 162 Phosphoserine.
 MOD_RES 166 166 Phosphoserine.
 MOD_RES 233 233 Phosphoserine.
 MOD_RES 241 241 Phosphoserine.
 MOD_RES 253 253 Phosphoserine.
 MOD_RES 260 260 Phosphoserine.
 MOD_RES 299 299 Phosphoserine.
 MOD_RES 306 306 Phosphoserine.
 CROSSLNK 250 250 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ribonucleoprotein; RNA-binding; Spliceosome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 306 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0045120; C:pronucleus; IEA:Compara.
 GO:0003729; F:mRNA binding; IEA:Compara.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; NAS:HGNC.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB. 

Interpro

 IPR017347; hnRNP_C_Raly.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS