CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005891
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tyrosine-protein kinase receptor UFO 
Protein Synonyms/Alias
 AXL oncogene 
Gene Name
 AXL 
Gene Synonyms/Alias
 UFO 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
514LGISEELKEKLRDVMubiquitination[1]
531RHKVALGKTLGEGEFubiquitination[1]
657GMEYLSTKRFIHRDLubiquitination[1]
705RIAKMPVKWIAIESLubiquitination[1]
760LRQGNRLKQPADCLDubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor. 
Sequence Annotation
 DOMAIN 27 128 Ig-like C2-type 1.
 DOMAIN 139 222 Ig-like C2-type 2.
 DOMAIN 225 332 Fibronectin type-III 1.
 DOMAIN 333 427 Fibronectin type-III 2.
 DOMAIN 536 807 Protein kinase.
 NP_BIND 542 550 ATP (By similarity).
 REGION 26 92 Interaction with GAS6.
 ACT_SITE 672 672 Proton acceptor (By similarity).
 BINDING 567 567 ATP (By similarity).
 MOD_RES 703 703 Phosphotyrosine; by autocatalysis (By
 MOD_RES 779 779 Phosphotyrosine; by autocatalysis.
 MOD_RES 821 821 Phosphotyrosine; by autocatalysis.
 MOD_RES 866 866 Phosphotyrosine; by autocatalysis.
 MOD_RES 884 884 Phosphoserine.
 CARBOHYD 43 43 N-linked (GlcNAc...) (Potential).
 CARBOHYD 157 157 N-linked (GlcNAc...) (Potential).
 CARBOHYD 198 198 N-linked (GlcNAc...) (Potential).
 CARBOHYD 339 339 N-linked (GlcNAc...) (Potential).
 CARBOHYD 345 345 N-linked (GlcNAc...) (Potential).
 CARBOHYD 401 401 N-linked (GlcNAc...) (Potential).
 DISULFID 56 117 By similarity.
 DISULFID 160 205 By similarity.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Differentiation; Disulfide bond; Glycoprotein; Immunity; Immunoglobulin domain; Innate immunity; Kinase; Membrane; Nucleotide-binding; Oncogene; Phosphoprotein; Polymorphism; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 894 AA 
Protein Sequence
MAWRCPRMGR VPLAWCLALC GWACMAPRGT QAEESPFVGN PGNITGARGL TGTLRCQLQV 60
QGEPPEVHWL RDGQILELAD STQTQVPLGE DEQDDWIVVS QLRITSLQLS DTGQYQCLVF 120
LGHQTFVSQP GYVGLEGLPY FLEEPEDRTV AANTPFNLSC QAQGPPEPVD LLWLQDAVPL 180
ATAPGHGPQR SLHVPGLNKT SSFSCEAHNA KGVTTSRTAT ITVLPQQPRN LHLVSRQPTE 240
LEVAWTPGLS GIYPLTHCTL QAVLSNDGMG IQAGEPDPPE EPLTSQASVP PHQLRLGSLH 300
PHTPYHIRVA CTSSQGPSSW THWLPVETPE GVPLGPPENI SATRNGSQAF VHWQEPRAPL 360
QGTLLGYRLA YQGQDTPEVL MDIGLRQEVT LELQGDGSVS NLTVCVAAYT AAGDGPWSLP 420
VPLEAWRPVK EPSTPAFSWP WWYVLLGAVV AAACVLILAL FLVHRRKKET RYGEVFEPTV 480
ERGELVVRYR VRKSYSRRTT EATLNSLGIS EELKEKLRDV MVDRHKVALG KTLGEGEFGA 540
VMEGQLNQDD SILKVAVKTM KIAICTRSEL EDFLSEAVCM KEFDHPNVMR LIGVCFQGSE 600
RESFPAPVVI LPFMKHGDLH SFLLYSRLGD QPVYLPTQML VKFMADIASG MEYLSTKRFI 660
HRDLAARNCM LNENMSVCVA DFGLSKKIYN GDYYRQGRIA KMPVKWIAIE SLADRVYTSK 720
SDVWSFGVTM WEIATRGQTP YPGVENSEIY DYLRQGNRLK QPADCLDGLY ALMSRCWELN 780
PQDRPSFTEL REDLENTLKA LPPAQEPDEI LYVNMDEGGG YPEPPGAAGG ADPPTQPDPK 840
DSCSCLTAAE VHPAGRYVLC PSTTPSPAQP ADRGSPAAPG QEDGA 885 
Gene Ontology
 GO:0009986; C:cell surface; IDA:UniProtKB.
 GO:0005615; C:extracellular space; IDA:UniProtKB.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0001786; F:phosphatidylserine binding; IDA:UniProtKB.
 GO:0004714; F:transmembrane receptor protein tyrosine kinase activity; TAS:ProtInc.
 GO:0043277; P:apoptotic cell clearance; IEA:Compara.
 GO:0001974; P:blood vessel remodeling; IEA:Compara.
 GO:0048469; P:cell maturation; IEP:UniProtKB.
 GO:0031668; P:cellular response to extracellular stimulus; IEA:Compara.
 GO:0035457; P:cellular response to interferon-alpha; IDA:UniProtKB.
 GO:0071222; P:cellular response to lipopolysaccharide; IDA:UniProtKB.
 GO:0097028; P:dendritic cell differentiation; IEP:UniProtKB.
 GO:0007167; P:enzyme linked receptor protein signaling pathway; IEA:Compara.
 GO:0034101; P:erythrocyte homeostasis; IEA:Compara.
 GO:0021885; P:forebrain cell migration; IEA:Compara.
 GO:0006954; P:inflammatory response; IEA:Compara.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0001779; P:natural killer cell differentiation; IEA:Compara.
 GO:2000669; P:negative regulation of dendritic cell apoptotic process; IDA:UniProtKB.
 GO:0032689; P:negative regulation of interferon-gamma production; IDA:UniProtKB.
 GO:0051250; P:negative regulation of lymphocyte activation; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0032720; P:negative regulation of tumor necrosis factor production; IEA:Compara.
 GO:0001764; P:neuron migration; IEA:Compara.
 GO:0042698; P:ovulation cycle; IEA:Compara.
 GO:0006909; P:phagocytosis; IDA:UniProtKB.
 GO:0030168; P:platelet activation; IEA:Compara.
 GO:0001961; P:positive regulation of cytokine-mediated signaling pathway; IDA:UniProtKB.
 GO:0032825; P:positive regulation of natural killer cell differentiation; IDA:UniProtKB.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0043491; P:protein kinase B signaling cascade; IEA:Compara.
 GO:0032940; P:secretion by cell; IEA:Compara.
 GO:0007283; P:spermatogenesis; IEA:Compara.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IEA:Compara.
 GO:0060068; P:vagina development; IEA:Compara. 
Interpro
 IPR003961; Fibronectin_type3.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003599; Ig_sub.
 IPR013106; Ig_V-set.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom. 
Pfam
 PF00041; fn3
 PF07714; Pkinase_Tyr
 PF07686; V-set 
SMART
 SM00060; FN3
 SM00409; IG
 SM00219; TyrKc 
PROSITE
 PS50853; FN3
 PS50835; IG_LIKE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR 
PRINTS
 PR00109; TYRKINASE.