UniProt Accession

 DUO1_YEAST; P53168; D6VU80 

Genbank Protein ID

 Z72583; AY558484; BK006941 

Genbank Nucleotide ID

 CAA96764.1; AAS56810.1; DAA08041.1 

Protein Name

 DASH complex subunit DUO1 

Protein Synonyms/Alias

 Death upon overproduction protein 1; Outer kinetochore protein DUO1 

Gene Name

 DUO1 

Gene Synonyms/Alias

 YGL061C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
33	NLNNTTNKFPKSTGG	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. 

Sequence Annotation

  

Keyword

 Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 247 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0042729; C:DASH complex; IDA:SGD.
 GO:0072686; C:mitotic spindle; IEA:InterPro.
 GO:0005876; C:spindle microtubule; IDA:SGD.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0005200; F:structural constituent of cytoskeleton; IPI:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0030472; P:mitotic spindle organization in nucleus; IPI:SGD.
 GO:0031110; P:regulation of microtubule polymerization or depolymerization; IDA:SGD. 

Interpro

 IPR013960; DASH_Duo1. 

Pfam

 PF08651; DASH_Duo1 

SMART

  

PROSITE

  

PRINTS