UniProt Accession

 SEQA_ECOLI; P0AFY8; P36658 

Genbank Protein ID

 U07651; U00096; AP009048 

Genbank Nucleotide ID

 AAA19855.1; AAC73781.1; BAA35336.1 

Protein Name

 Negative modulator of initiation of replication 

Protein Synonyms/Alias

  

Gene Name

 seqA 

Gene Synonyms/Alias

 b0687; JW0674 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
19	SYIASHTKHIGESAS	acetylation	[1]
34	DILRRMLKFSAASQP	acetylation	[1]
47	QPAAPVTKEVRVASP	acetylation	[1]
60	SPAIVEAKPVKTIKD	acetylation	[1]
63	IVEAKPVKTIKDKVR	acetylation	[1]
85	SDEYAEQKRAVNRFM	acetylation	[1]
130	ADEQTLLKNGNQTKP	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Negative regulator of replication initiation, which contributes to regulation of DNA replication and ensures that replication initiation occurs exactly once per chromosome per cell cycle. Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re- initiation at newly replicated origins. Repression is relieved when the region becomes fully methylated. Can also bind to hemimethylated GATC sequences outside of oriC region. Binds, with less affinity, to fully methylated GATC sites and affects timing of replication. May play a role in chromosome organization and gene regulation. 

Sequence Annotation

 REGION 87 88 Interaction with DNA.
 REGION 116 120 Interaction with DNA.
 REGION 150 156 Interaction with DNA.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 181 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:HAMAP.
 GO:0032297; P:negative regulation of DNA-dependent DNA replication initiation; IMP:EcoCyc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro. 

Interpro

 IPR013321; Arc_rbn_hlx_hlx.
 IPR005621; Regulation_modulator_SeqA.
 IPR010985; Ribbon_hlx_hlx.
 IPR026577; SeqA_DNA-bd_C. 

Pfam

 PF03925; SeqA 

SMART

  

PROSITE

  

PRINTS