CPLM-004811

Genbank Nucleotide ID

AP-1-like transcription factor YAP1

Protein Synonyms/Alias

Phenanthroline resistance protein PAR1; Pleiotropic drug resistance protein PDR4

PAR1; PDR4; SNQ3; YML007W; YM9571.12

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
221	MPINQTQKKLSDATD	ubiquitination	[1]
276	GDDGSNSKTKNLDSN	ubiquitination	[1]
591	DNDVVPSKEGSLLRC	ubiquitination	[1]

[1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]

Functional Description

Transcription activator involved in oxidative stress response and redox homeostasis. Regulates the transcription of genes encoding antioxidant enzymes and components of the cellular thiol-reducing pathways, including the thioredoxin system (TRX2, TRR1), the glutaredoxin system (GSH1, GLR1), superoxide dismutase (SOD1, SOD2), glutathione peroxidase (GPX2), and thiol-specific peroxidases (TSA1, AHP1). The induction of some of these genes requires the cooperative action of both, YAP1 and SKN7. YAP1 preferentially binds to promoters with the core binding site 5'- TTA[CG]TAA-3'. Activity of YAP1 is controlled through oxidation of specific cysteine residues resulting in the alteration of its subcellular location. Oxidative stress (as well as carbon stress, but not increased temperature, acidic pH, or ionic stress) induces nuclear accumulation and as a result YAP1 transcriptional activity. Nuclear export is restored when disulfide bonds are reduced by thioredoxin (TRX2), whose expression is controlled by YAP1, providing a mechanism for negative autoregulation. When overexpressed, YAP1 confers pleiotropic drug-resistance and increases cellular tolerance to cadmium, iron chelators and zinc.

DOMAIN 64 127 bZIP.
REGION 67 90 Basic motif (By similarity).
REGION 92 120 Leucine-zipper (By similarity).
REGION 220 378 Transcription activation 1.
REGION 430 537 Transcription activation 2.
MOTIF 5 16 Nuclear localization signal.
MOTIF 50 59 Nuclear localization signal.
MOTIF 614 621 Nuclear export signal.
MOD_RES 9 9 Phosphoserine.
MOD_RES 14 14 Phosphoserine.
MOD_RES 17 17 Phosphoserine.
MOD_RES 165 165 Phosphothreonine.
MOD_RES 204 204 Phosphoserine.
MOD_RES 372 372 Phosphoserine.
MOD_RES 528 528 Phosphoserine.
DISULFID 303 598 In nuclear retained form.
DISULFID 310 629 In nuclear retained form.
DISULFID 598 629 In nuclear retained form; alternate.
DISULFID 598 620 In nuclear retained form; alternate.
DISULFID 598 598 Interchain (with C-36 in HYR1);
DISULFID 620 629 In nuclear retained form; alternate.

3D-structure; Activator; Cadmium resistance; Complete proteome; Cytoplasm; Disulfide bond; DNA-binding; Nucleus; Oxidation; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:SGD.
GO:0005634; C:nucleus; IDA:SGD.
GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:SGD.
GO:0061395; P:positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance; IMP:SGD.
GO:0043619; P:regulation of transcription from RNA polymerase II promoter in response to oxidative stress; IDA:SGD.
GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
GO:0042493; P:response to drug; IMP:SGD.
GO:0009408; P:response to heat; IMP:SGD.
GO:0010038; P:response to metal ion; IMP:SGD.
GO:0000304; P:response to singlet oxygen; IMP:SGD.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR004827; bZIP.
IPR013910; TF_PAP1.
IPR023167; Yap1_redox_dom.

PF00170; bZIP_1
PF08601; PAP1

PS50217; BZIP
PS00036; BZIP_BASIC