CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001874
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myc proto-oncogene protein 
Protein Synonyms/Alias
 Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64 
Gene Name
 MYC 
Gene Synonyms/Alias
 BHLHE39 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67PSEDIWKKFELLPTPubiquitination[1]
163AAKLVSEKLASYQAAubiquitination[1, 2, 3, 4, 5]
172ASYQAARKDSGSPNPubiquitination[2]
404PELENNEKAPKVVILubiquitination[1, 4]
413PKVVILKKATAYILSubiquitination[1]
427SVQAEEQKLISEEDLubiquitination[4]
445RREQLKHKLEQLRNSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. 
Sequence Annotation
 DOMAIN 354 406 bHLH.
 REGION 413 434 Leucine-zipper.
 MOD_RES 6 6 Phosphoserine.
 MOD_RES 8 8 Phosphothreonine; by RAF; in vitro.
 MOD_RES 58 58 Phosphothreonine; by GSK3; alternate.
 MOD_RES 62 62 Phosphoserine; by DYRK2, GSK3 and CDK2.
 MOD_RES 71 71 Phosphoserine.
 MOD_RES 143 143 N6-acetyllysine; by PCAF.
 MOD_RES 148 148 N6-acetyllysine.
 MOD_RES 157 157 N6-acetyllysine; by PCAF.
 MOD_RES 161 161 Phosphoserine.
 MOD_RES 275 275 N6-acetyllysine; by PCAF.
 MOD_RES 317 317 N6-acetyllysine; by PCAF.
 MOD_RES 323 323 N6-acetyllysine; by PCAF.
 MOD_RES 329 329 Phosphoserine; by PIM2; in vitro (By
 MOD_RES 371 371 N6-acetyllysine; by PCAF.
 CARBOHYD 58 58 O-linked (GlcNAc); alternate.  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Chromosomal rearrangement; Complete proteome; DNA-binding; Glycoprotein; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 439 AA 
Protein Sequence
MDFFRVVENQ QPPATMPLNV SFTNRNYDLD YDSVQPYFYC DEEENFYQQQ QQSELQPPAP 60
SEDIWKKFEL LPTPPLSPSR RSGLCSPSYV AVTPFSLRGD NDGGGGSFST ADQLEMVTEL 120
LGGDMVNQSF ICDPDDETFI KNIIIQDCMW SGFSAAAKLV SEKLASYQAA RKDSGSPNPA 180
RGHSVCSTSS LYLQDLSAAA SECIDPSVVF PYPLNDSSSP KSCASQDSSA FSPSSDSLLS 240
STESSPQGSP EPLVLHEETP PTTSSDSEEE QEDEEEIDVV SVEKRQAPGK RSESGSPSAG 300
GHSKPPHSPL VLKRCHVSTH QHNYAAPPST RKDYPAAKRV KLDSVRVLRQ ISNNRKCTSP 360
RSSDTEENVK RRTHNVLERQ RRNELKRSFF ALRDQIPELE NNEKAPKVVI LKKATAYILS 420
VQAEEQKLIS EEDLLRKRRE QLKHKLEQLR NSCA 454 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; IDA:UniProtKB.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0005819; C:spindle; IEA:Compara.
 GO:0070888; F:E-box binding; IDA:UniProtKB.
 GO:0032403; F:protein complex binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0001658; P:branching involved in ureteric bud morphogenesis; ISS:UniProtKB.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IDA:UniProtKB.
 GO:0007050; P:cell cycle arrest; IDA:UniProtKB.
 GO:0006879; P:cellular iron ion homeostasis; IDA:UniProtKB.
 GO:0035690; P:cellular response to drug; IDA:UniProtKB.
 GO:0034644; P:cellular response to UV; IEP:UniProtKB.
 GO:0006338; P:chromatin remodeling; IDA:UniProtKB.
 GO:0006112; P:energy reserve metabolic process; NAS:UniProtKB.
 GO:0044346; P:fibroblast apoptotic process; TAS:UniProtKB.
 GO:0000165; P:MAPK cascade; IMP:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0051782; P:negative regulation of cell division; IDA:UniProtKB.
 GO:0048147; P:negative regulation of fibroblast proliferation; IDA:UniProtKB.
 GO:0045656; P:negative regulation of monocyte differentiation; IMP:UniProtKB.
 GO:0032873; P:negative regulation of stress-activated MAPK cascade; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0015671; P:oxygen transport; NAS:UniProtKB.
 GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:UniProtKB.
 GO:2000573; P:positive regulation of DNA biosynthetic process; IMP:UniProtKB.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IDA:UniProtKB.
 GO:0048146; P:positive regulation of fibroblast proliferation; IDA:UniProtKB.
 GO:0090096; P:positive regulation of metanephric cap mesenchymal cell proliferation; ISS:UniProtKB.
 GO:2001022; P:positive regulation of response to DNA damage stimulus; IDA:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0032204; P:regulation of telomere maintenance; IMP:BHF-UCL.
 GO:0006974; P:response to DNA damage stimulus; IDA:UniProtKB.
 GO:0010332; P:response to gamma radiation; IDA:UniProtKB.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR011598; bHLH_dom.
 IPR003327; Myc-LZ.
 IPR002418; Tscrpt_reg_Myc.
 IPR012682; Tscrpt_reg_Myc_N. 
Pfam
 PF00010; HLH
 PF02344; Myc-LZ
 PF01056; Myc_N 
SMART
 SM00353; HLH 
PROSITE
 PS50888; BHLH 
PRINTS
 PR00044; LEUZIPPRMYC.