CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017868
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear pore complex protein Nup85 
Protein Synonyms/Alias
 85 kDa nucleoporin; FROUNT; Nucleoporin Nup85; Pericentrin-1 
Gene Name
 Nup85 
Gene Synonyms/Alias
 Pcnt1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
47NQTGKSEKVPSCPFIubiquitination[1]
59PFIYIIRKDVDVYSQubiquitination[1]
92IEEELSGKSRKAQLVacetylation[2]
92IEEELSGKSRKAQLVubiquitination[1]
218EARQMLAKEADANPSubiquitination[1]
631DDDIETTKVEMLRLAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine (By similarity).  
Keyword
 Acetylation; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Protein transport; Reference proteome; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 656 AA 
Protein Sequence
MEELDCEPAV TWIPGVNSKK KQMCFDWGPG EMLLCETSFN QTGKSEKVPS CPFIYIIRKD 60
VDVYSQILRK LFNESHGIFV GLQKIEEELS GKSRKAQLVR VSKNYRSVIR ACMEEMHQVA 120
IAAKDPASGR QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL 180
SADVLGGDNP SKHENFWDLV TVLVLQGRLD EARQMLAKEA DANPSCAGMC RVLGDLMRTM 240
PILSPGNTQT LTELELKWQH WREECERHLQ DNTFAANPRL ESLCKIMLGD EAALLEQKEL 300
LSNWYHFLVT RLLYSNPTVK PIDLHFYAQS SLDMFLGGES SPEPLDNILM AAFEFDIHQV 360
IKECSIALSN WWFVAHLTDL LDHCRLLQSH NLYFGSNMRE FLLLEYASGL FAHHSLWQLG 420
VDYFDYCPEL GRVSLELHIE RIPLNTEQKA LKVLRICEQR QMTEQVKSIC KILAMKAVRN 480
NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGSAMM LSDRLTFLGK 540
YREFHRLYGE KRFGDAASLL LSLMTSQIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL 600
MRCLEDLASG RPECGEPDAQ RLQDDDIETT KVEMLRLALA RNLARAIIRE GSLEGS 656 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; ISA:MGI.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0031080; C:nuclear pore outer ring; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; ISA:MGI.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0031727; F:CCR2 chemokine receptor binding; ISA:MGI.
 GO:0019221; P:cytokine-mediated signaling pathway; ISA:MGI.
 GO:0030032; P:lamellipodium assembly; IDA:MGI.
 GO:0048246; P:macrophage chemotaxis; IDA:MGI.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR011502; Nucleoporin_Nup85. 
Pfam
 PF07575; Nucleopor_Nup85 
SMART
  
PROSITE
  
PRINTS