CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023160
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA helicase INO80 
Protein Synonyms/Alias
 hINO80; INO80 complex subunit A; Putative DNA helicase INO80 complex homolog 1 
Gene Name
 INO80 
Gene Synonyms/Alias
 INO80A; INOC1; KIAA1259 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
118GNLYNFSKLKKSRKWacetylation[1]
486AALRAANKSGTGFGEubiquitination[2]
1317KEDELDGKRRKEGVNubiquitination[3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle. 
Sequence Annotation
 DOMAIN 280 405 DBINO.
 DOMAIN 530 701 Helicase ATP-binding.
 DOMAIN 1105 1260 Helicase C-terminal.
 NP_BIND 543 550 ATP (By similarity).
 REGION 1 266 Assembles INO80 complex module with
 REGION 212 526 Assembles INO80 complex module consisting
 REGION 521 1556 Assembles INO80 complex module consisting
 MOD_RES 118 118 N6-acetyllysine.
 MOD_RES 1512 1512 Phosphoserine.  
Keyword
 Acetylation; Actin-binding; ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Microtubule; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1556 AA 
Protein Sequence
MASELGARDD GGCTELAKPL YLQYLERALR LDHFLRQTSA IFNRNISSDD SEDGLDDSNP 60
LLPQSGDPLI QVKEEPPNSL LGETSGAGSS GMLNTYSLNG VLQSESKCDK GNLYNFSKLK 120
KSRKWLKSIL LSDESSEADS QSEDDDEEEL NLSREELHNM LRLHKYKKLH QNKYSKDKEL 180
QQYQYYSAGL LSTYDPFYEQ QRHLLGPKKK KFKEEKKLKA KLKKVKKKRR RDEELSSEES 240
PRRHHHQTKV FAKFSHDAPP PGTKKKHLSI EQLNARRRKV WLSIVKKELP KANKQKASAR 300
NLFLTNSRKL AHQCMKEVRR AALQAQKNCK ETLPRARRLT KEMLLYWKKY EKVEKEHRKR 360
AEKEALEQRK LDEEMREAKR QQRKLNFLIT QTELYAHFMS RKRDMGHDGI QEEILRKLED 420
SSTQRQIDIG GGVVVNITQE DYDSNHFKAQ ALKNAENAYH IHQARTRSFD EDAKESRAAA 480
LRAANKSGTG FGESYSLANP SIRAGEDIPQ PTIFNGKLKG YQLKGMNWLA NLYEQGINGI 540
LADEMGLGKT VQSIALLAHL AERENIWGPF LIISPASTLN NWHQEFTRFV PKFKVLPYWG 600
NPHDRKVIRR FWSQKTLYTQ DAPFHVVITS YQLVVQDVKY FQRVKWQYMV LDEAQALKSS 660
SSVRWKILLQ FQCRNRLLLT GTPIQNTMAE LWALLHFIMP TLFDSHEEFN EWFSKDIESH 720
AENKSAIDEN QLSRLHMILK PFMLRRIKKD VENELSDKIE ILMYCQLTSR QKLLYQALKN 780
KISIEDLLQS SMGSTQQAQN TTSSLMNLVM QFRKVCNHPE LFERQETWSP FHISLKPYHI 840
SKFIYRHGQI RVFNHSRDRW LRVLSPFAPD YIQRSLFHRK GINEESCFSF LRFIDISPAE 900
MANLMLQGLL ARWLALFLSL KASYRLHQLR SWGAPEGESH QRYLRNKDFL LGVNFPLSFP 960
NLCSCPLLKS LVFSSHCKAV SGYSDQVVHQ RRSATSSLRR CLLTELPSFL CVASPRVTAV 1020
PLDSYCNDRS AEYERRVLKE GGSLAAKQCL LNGAPELAAD WLNRRSQFFP EPAGGLWSIR 1080
PQNGWSFIRI PGKESLITDS GKLYALDVLL TRLKSQGHRV LIYSQMTRMI DLLEEYMVYR 1140
KHTYMRLDGS SKISERRDMV ADFQNRNDIF VFLLSTRAGG LGINLTAADT VIFYDSDWNP 1200
TVDQQAMDRA HRLGQTKQVT VYRLICKGTI EERILQRAKE KSEIQRMVIS GGNFKPDTLK 1260
PKEVVSLLLD DEELEKKLRL RQEEKRQQEE TNRVKERKRK REKYAEKKKK EDELDGKRRK 1320
EGVNLVIPFV PSADNSNLSA DGDDSFISVD SAMPSPFSEI SISSELHTGS IPLDESSSDM 1380
LVIVDDPASS APQSRATNSP ASITGSVSDT VNGISIQEMP AAGRGHSARS RGRPKGSGST 1440
AKGAGKGRSR KSTAGSAAAM AGAKAGAAAA SAAAYAAYGY NVSKGISASS PLQTSLVRPA 1500
GLADFGPSSA SSPLSSPLSK GNNVPGNPKN LHMTSSLAPD SLVRKQGKGT NPSGGR 1556 
Gene Ontology
 GO:0031011; C:Ino80 complex; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0043014; F:alpha-tubulin binding; IMP:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0003678; F:DNA helicase activity; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0071479; P:cellular response to ionizing radiation; IMP:UniProtKB.
 GO:0034644; P:cellular response to UV; IMP:UniProtKB.
 GO:0006338; P:chromatin remodeling; IDA:UniProtKB.
 GO:0000724; P:double-strand break repair via homologous recombination; IMP:UniProtKB.
 GO:0000070; P:mitotic sister chromatid segregation; IMP:UniProtKB.
 GO:0030307; P:positive regulation of cell growth; IMP:UniProtKB.
 GO:0010571; P:positive regulation of nuclear cell cycle DNA replication; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:2000045; P:regulation of G1/S transition of mitotic cell cycle; IMP:UniProtKB.
 GO:0051225; P:spindle assembly; IMP:UniProtKB.
 GO:0070914; P:UV-damage excision repair; IMP:UniProtKB. 
Interpro
 IPR020838; DBINO.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF13892; DBINO
 PF00271; Helicase_C
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51413; DBINO
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS