CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030539
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Thimet oligopeptidase 
Protein Synonyms/Alias
 cDNA FLJ36073 fis, clone TESTI2019697, highly similar to THIMET OLIGOPEPTIDASE (EC 3.4.24.15) 
Gene Name
 THOP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
49VPRELLEKLIESRQAacetylation[1]
49VPRELLEKLIESRQAubiquitination[1, 2, 3, 4, 5]
70LRQIVLAKVDQALHTubiquitination[3, 5, 6]
139DMFHTRFKQEGVLNSubiquitination[5]
236RFLGRDPKQDAFLLSubiquitination[5]
244QDAFLLSKGLQVGGCubiquitination[5]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 258 AA 
Protein Sequence
MFSGTHVERD FVEAPSQMLE NWVWEQEPLL RMSRHYRTGS AVPRELLEKL IESRQANTGL 60
FNLRQIVLAK VDQALHTQTD ADPAEEYARL CQEILGVPAT PGTNMPATFG HLAGGYDAQY 120
YGYLWSEVYS MDMFHTRFKQ EGVLNSKFPH YEVRPLRHVS LCLPLTWCDP GSGQPPESLT 180
RNRWLPALRA GPALPGCNIA LGSLQVGMDY RSCILRPGGS EDASAMLRRF LGRDPKQDAF 240
LLSKGLQVGG CEPEPQVC 258 
Gene Ontology
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR024079; MetalloPept_cat_dom.
 IPR024077; Neurolysin/TOP_dom2.
 IPR001567; Pept_M3A_M3B. 
Pfam
 PF01432; Peptidase_M3 
SMART
  
PROSITE
  
PRINTS