CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000396
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Axin-1 
Protein Synonyms/Alias
 Axis inhibition protein 1; hAxin 
Gene Name
 AXIN1 
Gene Synonyms/Alias
 AXIN 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
857FEEKIIGKVEKVD**sumoylation[1]
860KIIGKVEKVD*****sumoylation[1]
Reference
 [1] SUMO-1 modification of the C-terminal KVEKVD of Axin is required for JNK activation but has no effect on Wnt signaling.
 Rui HL, Fan E, Zhou HM, Xu Z, Zhang Y, Lin SC.
 J Biol Chem. 2002 Nov 8;277(45):42981-6. [PMID: 12223491] 
Functional Description
 Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt- independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. 
Sequence Annotation
 DOMAIN 88 211 RGS.
 DOMAIN 780 862 DIX.
 REGION 209 338 Interaction with TP53 (By similarity).
 REGION 348 433 Interaction with GSK3B (By similarity).
 REGION 434 502 Interaction with CTNNB1 (By similarity).
 REGION 507 757 Interaction with RNF111.
 REGION 575 789 Interaction with PPP2CA.
 REGION 677 752 Interaction with HIPK2 (By similarity).
 MOTIF 20 29 Tankyrase-binding motif.
 MOD_RES 75 75 Phosphoserine; by CK1.
 MOD_RES 77 77 Phosphoserine; by CK1.
 MOD_RES 217 217 Phosphoserine; by CK1.
 MOD_RES 469 469 Phosphoserine; by CK1.
 MOD_RES 481 481 Phosphothreonine; by GSK3-beta
 MOD_RES 486 486 Phosphoserine; by GSK3-beta (By
 MOD_RES 493 493 Phosphoserine (By similarity).
 CROSSLNK 857 857 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 860 860 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; ADP-ribosylation; Alternative splicing; Apoptosis; Cell membrane; Complete proteome; Cytoplasm; Developmental protein; Disease mutation; Isopeptide bond; Membrane; Nucleus; Phosphoprotein; Reference proteome; Tumor suppressor; Ubl conjugation; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 862 AA 
Protein Sequence
MNIQEQGFPL DLGASFTEDA PRPPVPGEEG ELVSTDPRPA SYSFCSGKGV GIKGETSTAT 60
PRRSDLDLGY EPEGSASPTP PYLKWAESLH SLLDDQDGIS LFRTFLKQEG CADLLDFWFA 120
CTGFRKLEPC DSNEEKRLKL ARAIYRKYIL DNNGIVSRQT KPATKSFIKG CIMKQLIDPA 180
MFDQAQTEIQ ATMEENTYPS FLKSDIYLEY TRTGSESPKV CSDQSSGSGT GKGISGYLPT 240
LNEDEEWKCD QDMDEDDGRD AAPPGRLPQK LLLETAAPRV SSSRRYSEGR EFRYGSWREP 300
VNPYYVNAGY ALAPATSAND SEQQSLSSDA DTLSLTDSSV DGIPPYRIRK QHRREMQESV 360
QVNGRVPLPH IPRTYRVPKE VRVEPQKFAE ELIHRLEAVQ RTREAEEKLE ERLKRVRMEE 420
EGEDGDPSSG PPGPCHKLPP APAWHHFPPR CVDMGCAGLR DAHEENPESI LDEHVQRVLR 480
TPGRQSPGPG HRSPDSGHVA KMPVALGGAA SGHGKHVPKS GAKLDAAGLH HHRHVHHHVH 540
HSTARPKEQV EAEATRRAQS SFAWGLEPHS HGARSRGYSE SVGAAPNASD GLAHSGKVGV 600
ACKRNAKKAE SGKSASTEVP GASEDAEKNQ KIMQWIIEGE KEISRHRRTG HGSSGTRKPQ 660
PHENSRPLSL EHPWAGPQLR TSVQPSHLFI QDPTMPPHPA PNPLTQLEEA RRRLEEEEKR 720
ASRAPSKQRY VQEVMRRGRA CVRPACAPVL HVVPAVSDME LSETETRSQR KVGGGSAQPC 780
DSIVVAYYFC GEPIPYRTLV RGRAVTLGQF KELLTKKGSY RYYFKKVSDE FDCGVVFEEV 840
REDEAVLPVF EEKIIGKVEK VD 862 
Gene Ontology
 GO:0030877; C:beta-catenin destruction complex; IDA:UniProtKB.
 GO:0005938; C:cell cortex; IBA:RefGenome.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IBA:RefGenome.
 GO:0005881; C:cytoplasmic microtubule; IBA:RefGenome.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016328; C:lateral plasma membrane; IDA:MGI.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0014069; C:postsynaptic density; IBA:RefGenome.
 GO:0070016; F:armadillo repeat domain binding; ISS:BHF-UCL.
 GO:0008013; F:beta-catenin binding; IDA:BHF-UCL.
 GO:0005096; F:GTPase activator activity; IBA:RefGenome.
 GO:0002039; F:p53 binding; IDA:MGI.
 GO:0032947; F:protein complex scaffold; IDA:BHF-UCL.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0019901; F:protein kinase binding; ISS:BHF-UCL.
 GO:0004871; F:signal transducer activity; IDA:BHF-UCL.
 GO:0007257; P:activation of JUN kinase activity; IBA:RefGenome.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0048320; P:axial mesoderm formation; IBA:RefGenome.
 GO:0060823; P:canonical Wnt receptor signaling pathway involved in neural plate anterior/posterior pattern formation; IBA:RefGenome.
 GO:0008219; P:cell death; IBA:RefGenome.
 GO:0043623; P:cellular protein complex assembly; IDA:BHF-UCL.
 GO:0071407; P:cellular response to organic cyclic compound; IBA:RefGenome.
 GO:0031122; P:cytoplasmic microtubule organization; IBA:RefGenome.
 GO:0007368; P:determination of left/right symmetry; IBA:RefGenome.
 GO:0009950; P:dorsal/ventral axis specification; IBA:RefGenome.
 GO:0048048; P:embryonic eye morphogenesis; IBA:RefGenome.
 GO:0060272; P:embryonic skeletal joint morphogenesis; IBA:RefGenome.
 GO:0021797; P:forebrain anterior/posterior pattern specification; IBA:RefGenome.
 GO:0071514; P:genetic imprinting; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0055001; P:muscle cell development; IBA:RefGenome.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IBA:RefGenome.
 GO:0045599; P:negative regulation of fat cell differentiation; IBA:RefGenome.
 GO:0051248; P:negative regulation of protein metabolic process; IEA:Compara.
 GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IEA:Compara.
 GO:0006913; P:nucleocytoplasmic transport; IEA:Compara.
 GO:0030910; P:olfactory placode formation; IBA:RefGenome.
 GO:0001743; P:optic placode formation; IBA:RefGenome.
 GO:0046330; P:positive regulation of JNK cascade; ISS:UniProtKB.
 GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IDA:BHF-UCL.
 GO:0010800; P:positive regulation of peptidyl-threonine phosphorylation; IDA:BHF-UCL.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0045732; P:positive regulation of protein catabolic process; IDA:BHF-UCL.
 GO:2000060; P:positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; ISS:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:BHF-UCL.
 GO:0030511; P:positive regulation of transforming growth factor beta receptor signaling pathway; IEA:Compara.
 GO:0051443; P:positive regulation of ubiquitin-protein ligase activity; IMP:BHF-UCL.
 GO:0036342; P:post-anal tail morphogenesis; IEA:Compara.
 GO:0030163; P:protein catabolic process; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; IEA:Compara.
 GO:0035412; P:regulation of catenin import into nucleus; IBA:RefGenome.
 GO:0007605; P:sensory perception of sound; IEA:Compara.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro.
 GO:0021881; P:Wnt receptor signaling pathway involved in forebrain neuron fate commitment; IBA:RefGenome.
 GO:0090244; P:Wnt receptor signaling pathway involved in somitogenesis; IBA:RefGenome. 
Interpro
 IPR014936; Axin_b-cat-bd.
 IPR001158; DIX.
 IPR000342; Regulat_G_prot_signal.
 IPR024066; Regulat_G_prot_signal_dom1.
 IPR016137; Regulat_G_prot_signal_superfam. 
Pfam
 PF08833; Axin_b-cat_bind
 PF00778; DIX
 PF00615; RGS 
SMART
 SM00021; DAX
 SM00315; RGS 
PROSITE
 PS50841; DIX
 PS50132; RGS 
PRINTS
 PR01301; RGSPROTEIN.