UniProt Accession

 MED7_HUMAN; O43513 

Genbank Protein ID

 AF031383; AF104251; BC005250 

Genbank Nucleotide ID

 AAC52115.1; AAD12720.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 7 

Protein Synonyms/Alias

 hMED7; Activator-recruited cofactor 34 kDa component; ARC34; Cofactor required for Sp1 transcriptional activation subunit 9; CRSP complex subunit 9; Mediator complex subunit 7; RNA polymerase transcriptional regulation mediator subunit 7 homolog; Transcriptional coactivator CRSP33 

Gene Name

 MED7 

Gene Synonyms/Alias

 ARC34; CRSP9 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
155	ETAERFQKHLERVIE	ubiquitination	[1, 2, 3]
185	SEAGMRVKTEPMDAD	ubiquitination	[2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 

Sequence Annotation

 MOD_RES 194 194 Phosphoserine.  

Keyword

 Activator; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 233 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; IEA:Compara.
 GO:0005667; C:transcription factor complex; IDA:MGI.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 

Interpro

 IPR009244; Mediatior_Med7. 

Pfam

 PF05983; Med7 

SMART

  

PROSITE

  

PRINTS