CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006992
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RING finger protein ETP1 
Protein Synonyms/Alias
 BRAP2 homolog; Ethanol tolerance protein 1 
Gene Name
 ETP1 
Gene Synonyms/Alias
 YHL010C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
43SIGEESIKSNNQDWQubiquitination[1]
369VQNEVDGKLVEVGGSubiquitination[1, 2, 3, 4, 5]
410EKSNSNKKDGELAANubiquitination[1, 2, 3, 4, 6]
450YELKLQEKDQTASDSubiquitination[1, 5]
527NKALEESKQDLEEQVubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [5] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [6] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538] 
Functional Description
 May act as a cytoplasmic retention protein with a role in regulating nuclear transport. Binds nuclear localization sequences in vitro. Needed to adapt efficiently to ethanol, either as sole carbon source or as cell stressor. Involved in ethanol- dependent transcriptional activation of several genes and ethanol- induced protein turnover of some proteins. 
Sequence Annotation
 ZN_FING 240 280 RING-type.
 ZN_FING 299 360 UBP-type.
 MOD_RES 376 376 Phosphoserine.
 CROSSLNK 369 369 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 410 410 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Cytoplasm; Isopeptide bond; Metal-binding; Phosphoprotein; Reference proteome; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MDQFEYIITL EFGNQNQVES AYQIFKSIPK KLKAKSIGEE SIKSNNQDWQ DWRVCDLEID 60
MITDFKNQTS KEEESDLITS QYLGHGIIRL FKLSNANNTL NEKEILTIPG DDTMICILFV 120
PTYFTVHDLL HFYIGDDIVN KQVSNFRILR NQQKGMGFNF TVLIKFRNAL DAKNFKEEFN 180
GKSFSRMDPE TCHVISVKEI VFQKKLFQRP AANEDFPYLL TDPFTVKKKK ELVKVELPTC 240
PVCLERMDSE TTGLVTIPCQ HTFHCQCLNK WKNSRCPVCR HSSLRLSRES LLKQAGDSAH 300
CATCGSTDNL WICLICGNVG CGRYNSKHAI KHYEETLHCF AMDIRTQRVW DYAGDNYVHR 360
LVQNEVDGKL VEVGGSGDDD NNDIGNSDEL QNVVYGNRSK NGEKSNSNKK DGELAANFLR 420
HREYHLEYVQ VLISQLESQR EYYELKLQEK DQTASDSSNV ESLKKSMEDL KLQFQVTQKE 480
WQKREMAQKS KLEEDMLVIE GLQANLDHLS KKQEQLEREN KALEESKQDL EEQVKDLMFY 540
LDSQEKFKDA DESVKEGTIL IQQPHGAAQA SKSKKKRNKN KKAGK 585 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008139; F:nuclear localization sequence binding; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0045471; P:response to ethanol; IMP:SGD. 
Interpro
 IPR011422; BRAP2.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR001607; Znf_UBP. 
Pfam
 PF07576; BRAP2
 PF13639; zf-RING_2
 PF02148; zf-UBP 
SMART
 SM00184; RING
 SM00290; ZnF_UBP 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2
 PS50271; ZF_UBP 
PRINTS