CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017930
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase Kist 
Protein Synonyms/Alias
 Kinase interacting with stathmin; PAM COOH-terminal interactor protein 2; P-CIP2; U2AF homology motif kinase 1 
Gene Name
 UHMK1 
Gene Synonyms/Alias
 KIS; KIST 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
54GSPPGALKQFLPPGTubiquitination[1]
183IDFGLSFKEGNQDVKubiquitination[1]
190KEGNQDVKYIQTDGYubiquitination[1, 2, 3]
251TVRSQEWKANSSAIIubiquitination[1]
265IDHIFASKAVVNAAIubiquitination[1, 3]
282YHLRDLIKSMLHDDPubiquitination[1, 2]
364VVSLLVPKENPGRGQubiquitination[1]
383YANAGDSKAAQKLLTubiquitination[1, 2, 4]
387GDSKAAQKLLTGRMFubiquitination[1, 5]
397TGRMFDGKFVVATFYubiquitination[1]
410FYPLSAYKRGYLYQTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity). 
Sequence Annotation
 DOMAIN 23 304 Protein kinase.
 DOMAIN 324 406 RRM.
 NP_BIND 29 37 ATP (By similarity).
 ACT_SITE 158 158 Proton acceptor (By similarity).
 BINDING 54 54 ATP (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; RNA-binding; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 419 AA 
Protein Sequence
MAGSGCAWGA EPPRFLEAFG RLWQVQSRLG SGSSASVYRV RCCGNPGSPP GALKQFLPPG 60
TTGAAASAAE YGFRKERAAL EQLQGHRNIV TLYGVFTIHF SPNVPSRCLL LELLDVSVSE 120
LLLYSSHQGC SMWMIQHCAR DVLEALAFLH HEGYVHADLK PRNILWSAEN ECFKLIDFGL 180
SFKEGNQDVK YIQTDGYRAP EAELQNCLAQ AGLQSDTECT SAVDLWSLGI ILLEMFSGMK 240
LKHTVRSQEW KANSSAIIDH IFASKAVVNA AIPAYHLRDL IKSMLHDDPS RRIPAEMALC 300
SPFFSIPFAP HIEDLVMLPT PVLRLLNVLD DDYLENEEEY EDVVEDVKEE CQKYGPVVSL 360
LVPKENPGRG QVFVEYANAG DSKAAQKLLT GRMFDGKFVV ATFYPLSAYK RGYLYQTLL 419 
Gene Ontology
 GO:0030424; C:axon; ISS:BHF-UCL.
 GO:0032839; C:dendrite cytoplasm; ISS:BHF-UCL.
 GO:0071598; C:neuronal ribonucleoprotein granule; ISS:BHF-UCL.
 GO:0005634; C:nucleus; IDA:HGNC.
 GO:0005524; F:ATP binding; IC:HGNC.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:HGNC.
 GO:0043021; F:ribonucleoprotein complex binding; ISS:BHF-UCL.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007050; P:cell cycle arrest; IMP:HGNC.
 GO:0031175; P:neuron projection development; ISS:BHF-UCL.
 GO:0018105; P:peptidyl-serine phosphorylation; ISS:UniProtKB.
 GO:0045948; P:positive regulation of translational initiation; ISS:BHF-UCL.
 GO:0046777; P:protein autophosphorylation; IDA:HGNC.
 GO:0046825; P:regulation of protein export from nucleus; IDA:HGNC. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000719; Prot_kinase_cat_dom.
 IPR000504; RRM_dom. 
Pfam
 PF00069; Pkinase 
SMART
 SM00360; RRM 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50102; RRM 
PRINTS