CPLM-009716

Genbank Nucleotide ID

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-5

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
11	SSSVAAMKKVVQQLR	ubiquitination	[1, 2]
12	SSVAAMKKVVQQLRL	ubiquitination	[2, 3]
27	EAGLNRVKVSQAAAD	ubiquitination	[2, 3, 4]
36	SQAAADLKQFCLQNA	ubiquitination	[5, 6, 7]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.

MOD_RES 2 2 N-acetylserine.
MOD_RES 2 2 Phosphoserine.
MOD_RES 65 65 Cysteine methyl ester (By similarity).
LIPID 65 65 S-geranylgeranyl cysteine (By

Acetylation; Cell membrane; Complete proteome; Direct protein sequencing; Lipoprotein; Membrane; Methylation; Phosphoprotein; Prenylation; Reference proteome; Transducer.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005834; C:heterotrimeric G-protein complex; NAS:UniProtKB.
GO:0003924; F:GTPase activity; NAS:UniProtKB.
GO:0004871; F:signal transducer activity; NAS:UniProtKB.
GO:0071377; P:cellular response to glucagon stimulus; TAS:Reactome.
GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
GO:0007186; P:G-protein coupled receptor signaling pathway; NAS:UniProtKB.
GO:0007268; P:synaptic transmission; TAS:Reactome.

IPR015898; G-protein_gamma-like_dom.
IPR001770; Gprotein-gamma.

PS50058; G_PROTEIN_GAMMA