UniProt Accession

 MAPK5_HUMAN; Q8IW41; B3KVA5; O60491; Q86X46; Q9BVX9; Q9UG86 

Genbank Protein ID

 AF032437; AK122767; CH471054; BC000833; BC041049; BC047284; AL110301 

Genbank Nucleotide ID

 AAC39863.1; BAG53717.1; EAW97981.1; AAH00833.1; AAH41049.1; AAH47284.2; CAB53747.1 

Protein Name

 MAP kinase-activated protein kinase 5 

Protein Synonyms/Alias

 MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; p38-regulated/activated protein kinase; PRAK 

Gene Name

 MAPKAPK5 

Gene Synonyms/Alias

 PRAK 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
123	QHRHFTEKQASQVTK	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras- induced senescence and phosphorylating p53/TP53. Involved in post- transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. 

Sequence Annotation

 DOMAIN 22 304 Protein kinase.
 NP_BIND 28 36 ATP (By similarity).
 ACT_SITE 148 148 Proton acceptor (By similarity).
 BINDING 51 51 ATP (By similarity).
 MOD_RES 115 115 Phosphoserine; by PKA (By similarity).
 MOD_RES 182 182 Phosphothreonine; by MAPK11, MAPK14,
 MOD_RES 212 212 Phosphoserine.  

Keyword

 Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 473 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004708; F:MAP kinase kinase activity; TAS:ProtInc.
 GO:0002039; F:p53 binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0032007; P:negative regulation of TOR signaling cascade; ISS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:0007265; P:Ras protein signal transduction; IDA:UniProtKB.
 GO:0006417; P:regulation of translation; IDA:UniProtKB.
 GO:0090400; P:stress-induced premature senescence; IDA:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR027442; MAPKAPK_C.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS