CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005388
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosome assembly protein 4 
Protein Synonyms/Alias
  
Gene Name
 RSA4 
Gene Synonyms/Alias
 YCR072C; YCR72C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
27REVAIIPKDLPNVSIacetylation[1]
256PRLASSSKDGTIKIWubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Involved in processing and efficient intra-nuclear transport or pre-60S ribosomal subunits. Forms a complex with REA1 which is essential for ATP-dependent dissociation of a group of nonribosomal factors from the pre-60S particle. 
Sequence Annotation
 REPEAT 141 181 WD 1.
 REPEAT 184 223 WD 2.
 REPEAT 227 273 WD 3.
 REPEAT 276 314 WD 4.
 REPEAT 352 396 WD 5.
 REPEAT 400 439 WD 6.
 REPEAT 442 481 WD 7.
 REPEAT 484 514 WD 8.  
Keyword
 Complete proteome; Nucleus; Reference proteome; Repeat; Ribosome biogenesis; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 515 AA 
Protein Sequence
MSTLIPPPSK KQKKEAQLPR EVAIIPKDLP NVSIKFQALD TGDNVGGALR VPGAISEKQL 60
EELLNQLNGT SDDPVPYTFS CTIQGKKASD PVKTIDITDN LYSSLIKPGY NSTEDQITLL 120
YTPRAVFKVK PVTRSSSAIA GHGSTILCSA FAPHTSSRMV TGAGDNTARI WDCDTQTPMH 180
TLKGHYNWVL CVSWSPDGEV IATGSMDNTI RLWDPKSGQC LGDALRGHSK WITSLSWEPI 240
HLVKPGSKPR LASSSKDGTI KIWDTVSRVC QYTMSGHTNS VSCVKWGGQG LLYSGSHDRT 300
VRVWDINSQG RCINILKSHA HWVNHLSLST DYALRIGAFD HTGKKPSTPE EAQKKALENY 360
EKICKKNGNS EEMMVTASDD YTMFLWNPLK STKPIARMTG HQKLVNHVAF SPDGRYIVSA 420
SFDNSIKLWD GRDGKFISTF RGHVASVYQV AWSSDCRLLV SCSKDTTLKV WDVRTRKLSV 480
DLPGHKDEVY TVDWSVDGKR VCSGGKDKMV RLWTH 515 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0000027; P:ribosomal large subunit assembly; IMP:SGD. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR012972; NLE.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08154; NLE
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.