CPLM-022449

Genbank Nucleotide ID

Methionine adenosyltransferase 2 subunit beta

Protein Synonyms/Alias

Methionine adenosyltransferase II beta; MAT II beta; Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase

TGR; MSTP045; Nbla02999; UNQ2435/PRO4995

Homo sapiens (Human)

Position	Peptide	Type	References
46	LLGRAVHKEFQQNNW	ubiquitination	[1]
209	DKVQFSNKSANMDHW	ubiquitination	[1, 2, 3, 4]
225	QRFPTHVKDVATVCR	ubiquitination	[2, 4]
245	RMLDPSIKGTFHWSG	ubiquitination	[2, 4]
299	NAQLDCSKLETLGIG	ubiquitination	[1]
316	TPFRIGIKESLWPFL	ubiquitination	[1, 2, 4, 5, 6]
326	LWPFLIDKRWRQTVF	ubiquitination	[1, 2, 3, 4, 6, 7]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Non-catalytic regulatory subunit of S-adenosylmethionine synthetase 2 (MAT2A), an enzyme that catalyzes the formation of S- adenosylmethionine from methionine and ATP. Regulates the activity of S-adenosylmethionine synthetase 2 by changing its kinetic properties, rendering the enzyme more susceptible to S- adenosylmethionine inhibition.

3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; One-carbon metabolism; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0048269; C:methionine adenosyltransferase complex; IDA:UniProtKB.
GO:0005739; C:mitochondrion; IEA:Compara.
GO:0005634; C:nucleus; IDA:LIFEdb.
GO:0008831; F:dTDP-4-dehydrorhamnose reductase activity; IEA:InterPro.
GO:0048270; F:methionine adenosyltransferase regulator activity; IDA:UniProtKB.
GO:0045226; P:extracellular polysaccharide biosynthetic process; IEA:InterPro.
GO:0032259; P:methylation; TAS:Reactome.
GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
GO:0006556; P:S-adenosylmethionine biosynthetic process; IDA:UniProtKB.
GO:0000096; P:sulfur amino acid metabolic process; TAS:Reactome.
GO:0006805; P:xenobiotic metabolic process; TAS:Reactome.

IPR005913; dTDP_dehydrorham_reduct.
IPR016040; NAD(P)-bd_dom.

PF04321; RmlD_sub_bind