CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001751
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADP-specific glutamate dehydrogenase 
Protein Synonyms/Alias
 NADP-GDH 
Gene Name
 gdhA 
Gene Synonyms/Alias
 b1761; JW1750 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
16SFLNHVQKRDPNQTEacetylation[1]
44PFLEQNPKYRQMSLLacetylation[2]
136GGSDFDPKGKSEGEVacetylation[2]
250VAQYAIEKAMEFGARacetylation[2]
276VDESGFTKEKLARLIacetylation[2]
394NAARLGWKAEKVDARacetylation[2]
397RLGWKAEKVDARLHHacetylation[1, 2]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. 
Sequence Annotation
 ACT_SITE 128 128 Proton donor (By similarity).
 BINDING 92 92 Substrate (By similarity).
 BINDING 113 113 Substrate (By similarity).
 BINDING 116 116 Substrate (By similarity).
 BINDING 167 167 Substrate; via carbonyl oxygen (By
 BINDING 211 211 NADP (By similarity).
 BINDING 242 242 NADP (By similarity).
 BINDING 380 380 Substrate (By similarity).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 447 AA 
Protein Sequence
MDQTYSLESF LNHVQKRDPN QTEFAQAVRE VMTTLWPFLE QNPKYRQMSL LERLVEPERV 60
IQFRVVWVDD RNQIQVNRAW RVQFSSAIGP YKGGMRFHPS VNLSILKFLG FEQTFKNALT 120
TLPMGGGKGG SDFDPKGKSE GEVMRFCQAL MTELYRHLGA DTDVPAGDIG VGGREVGFMA 180
GMMKKLSNNT ACVFTGKGLS FGGSLIRPEA TGYGLVYFTE AMLKRHGMGF EGMRVSVSGS 240
GNVAQYAIEK AMEFGARVIT ASDSSGTVVD ESGFTKEKLA RLIEIKASRD GRVADYAKEF 300
GLVYLEGQQP WSLPVDIALP CATQNELDVD AAHQLIANGV KAVAEGANMP TTIEATELFQ 360
QAGVLFAPGK AANAGGVATS GLEMAQNAAR LGWKAEKVDA RLHHIMLDIH HACVEHGGEG 420
EQTNYVQGAN IAGFVKVADA MLAQGVI 447 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IDA:EcoCyc.
 GO:0006537; P:glutamate biosynthetic process; IDA:EcoCyc. 
Interpro
 IPR006095; Glu/Leu/Phe/Val_DH.
 IPR006096; Glu/Leu/Phe/Val_DH_C.
 IPR006097; Glu/Leu/Phe/Val_DH_dimer_dom.
 IPR014362; Glu_DH.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00208; ELFV_dehydrog
 PF02812; ELFV_dehydrog_N 
SMART
 SM00839; ELFV_dehydrog 
PROSITE
 PS00074; GLFV_DEHYDROGENASE 
PRINTS
 PR00082; GLFDHDRGNASE.