CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012943
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aspartate--tRNA ligase, mitochondrial 
Protein Synonyms/Alias
 Aspartyl-tRNA synthetase; AspRS 
Gene Name
 Dars2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
380EGAKYLRKEDIEFIRacetylation[1]
388EDIEFIRKFAAHHFSacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 MOD_RES 218 218 Phosphothreonine.  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 652 AA 
Protein Sequence
MYLGSWLNRL GRGLSRPIGK TKQPIWGSLS RSLTLSSQRV PEFSSFVART NTCGELRSSH 60
LGQEVTLCGW IQYRRQNTFL VLRDCHGLVQ ILIPQDESAA SVRRTLCEAP VESVVRVSGT 120
VIARPLGQEN PKMPTGEIEI KAKTAELLNA CKKLPFEIKD FVKKTEALRL QYRYLDLRSS 180
QMQHNLRLRS QMVMKMREYL CTLHGFVDIE TPTLFKRTPG GAKEFLVPSR EPGRFYSLPQ 240
SPQQFKQLLM VGGLDRYFQV ARCYRDEGSR PDRQPEFTQI DIEMSFVDQT GIQHLVEGLL 300
HYSWPEDKDP LVAPFPSMTF AEALATYGTD KPDTRFGMKI VDISDVFRNT EIRFLQDALA 360
KPQGTVKAIC VHEGAKYLRK EDIEFIRKFA AHHFSQEVLP IFLNARKNWS SPFAKFITEE 420
ERLELTRLME IQEDDMVLLT AGQHEKACSL LGKLRLECAD LLETRGLALR DPALFSFLWV 480
LDFPLFLAKE ESPTELESAH HPFTAPHPGD IHLLYTEPEK VRGQHYDLVL NGNEIGGGSI 540
RIHDAQLQRY ILETLLKEDV KLLSHLLQAL DYGAPPHGGI ALGLDRLVCL VTGAPSIRDV 600
IAFPKSYRGH DLMSNAPDTV SPEDLKPYHI HVSWPTDSEE RASATPSKYL SS 652 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004815; F:aspartate-tRNA ligase activity; IEA:EC.
 GO:0050560; F:aspartate-tRNA(Asn) ligase activity; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro. 
Interpro
 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR004524; Asp-tRNA-ligase_IIb_bac/mt.
 IPR002312; Asp/Asn-tRNA-synth_IIb.
 IPR004115; GAD_dom.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 
Pfam
 PF02938; GAD
 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01042; TRNASYNTHASP.