UniProt Accession

 VATB_YEAST; P16140; D6VQC4; P32123 

Genbank Protein ID

 J04450; X75891; Z35996; AY693158; M83130; BK006936 

Genbank Nucleotide ID

 AAA66890.1; CAA53486.1; CAA85084.1; AAT93177.1; AAA30389.1; DAA07244.1 

Protein Name

 V-type proton ATPase subunit B 

Protein Synonyms/Alias

 V-ATPase subunit B; V-ATPase 57 kDa subunit; Vacuolar proton pump subunit B 

Gene Name

 VMA2 

Gene Synonyms/Alias

 VAT2; YBR127C; YBR1002 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
13	KELFAINKKAVEQGF	acetylation	[1]
14	ELFAINKKAVEQGFN	ubiquitination	[2, 3]
23	VEQGFNVKPRLNYNT	acetylation	[1]
23	VEQGFNVKPRLNYNT	ubiquitination	[3]
90	TSGIDVKKTTVEFTG	ubiquitination	[3]
125	RPIDNGPKVFAEDYL	ubiquitination	[3]
169	NSIARGQKIPIFSAS	ubiquitination	[3]
226	LETARFFKQDFEENG	acetylation	[1]
226	LETARFFKQDFEENG	ubiquitination	[3]
384	PSLSRLMKSAIGEGM	ubiquitination	[3]
394	IGEGMTRKDHGDVSN	ubiquitination	[3]
406	VSNQLYAKYAIGKDA	acetylation	[1]
411	YAKYAIGKDAAAMKA	ubiquitination	[3]
441	EFLEKFEKTFITQGA	acetylation	[1]
441	EFLEKFEKTFITQGA	ubiquitination	[3]
470	SLLRIYPKEMLNRIS	ubiquitination	[3]
479	MLNRISPKILDEFYD	acetylation	[1]
479	MLNRISPKILDEFYD	ubiquitination	[3]
508	TRSSGKKKDASQEES	ubiquitination	[2, 3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles. 

Sequence Annotation

 MOD_RES 4 4 Phosphoserine.
 MOD_RES 137 137 Phosphoserine.
 MOD_RES 503 503 Phosphoserine.
 MOD_RES 504 504 Phosphoserine.
 MOD_RES 511 511 Phosphoserine; by ATM or ATR.
 MOD_RES 515 515 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; Hydrogen ion transport; Hydrolase; Ion transport; Phosphoprotein; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 517 AA 

Protein Sequence

Gene Ontology

 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0000221; C:vacuolar proton-transporting V-type ATPase, V1 domain; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IMP:SGD.
 GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro.
 GO:0006874; P:cellular calcium ion homeostasis; IMP:SGD.
 GO:0007035; P:vacuolar acidification; IMP:SGD. 

Interpro

 IPR020003; ATPase_a/bsu_AS.
 IPR004100; ATPase_a/bsu_N.
 IPR000793; ATPase_F1/V1/A1-cplx_a/bsu_C.
 IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd.
 IPR005723; ATPase_V1-cplx_bsu.
 IPR027417; P-loop_NTPase.
 IPR022879; V-ATPase_su_B/beta. 

Pfam

 PF00006; ATP-synt_ab
 PF00306; ATP-synt_ab_C
 PF02874; ATP-synt_ab_N 

SMART

  

PROSITE

 PS00152; ATPASE_ALPHA_BETA 

PRINTS