CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010857
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Adenylyl cyclase-associated protein 1 
Protein Synonyms/Alias
 CAP 1 
Gene Name
 CAP1 
Gene Synonyms/Alias
 CAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
63AEYLKISKEIGGDVQacetylation[1, 2]
71EIGGDVQKHAEMVHTacetylation[3]
71EIGGDVQKHAEMVHTubiquitination[4]
81EMVHTGLKLERALLVacetylation[1, 2, 3, 5]
81EMVHTGLKLERALLVubiquitination[6]
100CQQPAENKLSDLLAPacetylation[3]
209TTGLAWSKTGPVAKEacetylation[3, 5]
298GPVRSGPKPFSAPKPacetylation[3]
328VLELEGKKWRVENQEubiquitination[6]
376SITVDNCKKLGLVFDacetylation[3]
398IINSKDVKVQVMGKVubiquitination[6]
412VPTISINKTDGCHAYacetylation[2, 3]
412VPTISINKTDGCHAYubiquitination[2, 4, 6]
Reference
 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity. 
Sequence Annotation
 DOMAIN 319 453 C-CAP/cofactor C-like.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 34 34 Phosphoserine.
 MOD_RES 81 81 N6-acetyllysine.
 MOD_RES 290 290 Phosphoserine.
 MOD_RES 295 295 Phosphoserine.
 MOD_RES 301 301 Phosphoserine.
 MOD_RES 307 307 Phosphothreonine.
 MOD_RES 308 308 Phosphoserine.
 MOD_RES 310 310 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Actin-binding; Alternative splicing; Cell membrane; Complete proteome; Direct protein sequencing; Membrane; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 475 AA 
Protein Sequence
MADMQNLVER LERAVGRLEA VSHTSDMHRG YADSPSKAGA APYVQAFDSL LAGPVAEYLK 60
ISKEIGGDVQ KHAEMVHTGL KLERALLVTA SQCQQPAENK LSDLLAPISE QIKEVITFRE 120
KNRGSKLFNH LSAVSESIQA LGWVAMAPKP GPYVKEMNDA AMFYTNRVLK EYKDVDKKHV 180
DWVKAYLSIW TELQAYIKEF HTTGLAWSKT GPVAKELSGL PSGPSAGSCP PPPPPCPPPP 240
PVSTISCSYE SASRSSLFAQ INQGESITHA LKHVSDDMKT HKNPALKAQS GPVRSGPKPF 300
SAPKPQTSPS PKRATKKEPA VLELEGKKWR VENQENVSNL VIEDTELKQV AYIYKCVNTT 360
LQIKGKINSI TVDNCKKLGL VFDDVVGIVE IINSKDVKVQ VMGKVPTISI NKTDGCHAYL 420
SKNSLDCEIV SAKSSEMNVL IPTEGGDFNE FPVPEQFKTL WNGQKLVTTV TEIAG 475 
Gene Ontology
 GO:0030864; C:cortical actin cytoskeleton; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0030036; P:actin cytoskeleton organization; IEA:Compara.
 GO:0007190; P:activation of adenylate cyclase activity; TAS:ProtInc.
 GO:0001667; P:ameboidal cell migration; IEA:Compara.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0007163; P:establishment or maintenance of cell polarity; TAS:ProtInc.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0002576; P:platelet degranulation; TAS:Reactome.
 GO:0006898; P:receptor-mediated endocytosis; IEA:Compara.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR001837; Adenylate_cyclase-assoc_CAP.
 IPR013912; Adenylate_cyclase-assoc_CAP_C.
 IPR013992; Adenylate_cyclase-assoc_CAP_N.
 IPR017901; C-CAP_CF_C-like.
 IPR016098; CAP/MinC_C.
 IPR018106; CAP_CS.
 IPR006599; CARP_motif. 
Pfam
 PF08603; CAP_C
 PF01213; CAP_N 
SMART
 SM00673; CARP 
PROSITE
 PS51329; C_CAP_COFACTOR_C
 PS01088; CAP_1
 PS01089; CAP_2 
PRINTS