UniProt Accession

 THIL_ECOLI; P0AGG0; O32380; P77785; Q2MC09 

Genbank Protein ID

 D17333; U82664; U00096; AP009048 

Genbank Nucleotide ID

 BAA21778.1; AAB40173.1; AAC73520.1; BAE76197.1 

Protein Name

 Thiamine-monophosphate kinase 

Protein Synonyms/Alias

 TMP kinase; Thiamine-phosphate kinase 

Gene Name

 thiL 

Gene Synonyms/Alias

 b0417; JW0407 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
181	RLQVADAKDADYLIK	acetylation	[1]
188	KDADYLIKRHLRPSP	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the ATP-dependent phosphorylation of thiamine- monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1. Can not use thiamine as substrate. Is highly specific for ATP as phosphate donor. 

Sequence Annotation

 NP_BIND 121 122 ATP (By similarity).
 METAL 30 30 Magnesium 3 (By similarity).
 METAL 30 30 Magnesium 4; via carbonyl oxygen (By
 METAL 45 45 Magnesium 4 (By similarity).
 METAL 46 46 Magnesium 1; via carbonyl oxygen (By
 METAL 47 47 Magnesium 1 (By similarity).
 METAL 47 47 Magnesium 2 (By similarity).
 METAL 75 75 Magnesium 2 (By similarity).
 METAL 75 75 Magnesium 3 (By similarity).
 METAL 75 75 Magnesium 4 (By similarity).
 METAL 122 122 Magnesium 1 (By similarity).
 METAL 212 212 Magnesium 3 (By similarity).
 METAL 215 215 Magnesium 5 (By similarity).
 BINDING 54 54 Substrate (By similarity).
 BINDING 146 146 ATP (By similarity).
 BINDING 214 214 ATP (By similarity).
 BINDING 263 263 Substrate (By similarity).
 BINDING 319 319 Substrate (By similarity).  

Keyword

 ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Thiamine biosynthesis; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 325 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0009030; F:thiamine-phosphate kinase activity; IDA:UniProtKB.
 GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009229; P:thiamine diphosphate biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR010918; AIR_synth_C_dom.
 IPR000728; AIR_synth_N_dom.
 IPR016188; PurM_N-like.
 IPR006283; ThiL. 

Pfam

 PF00586; AIRS
 PF02769; AIRS_C 

SMART

  

PROSITE

  

PRINTS