CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014484
UniProt Accession
Genbank Protein ID
 L35271 
Genbank Nucleotide ID
Protein Name
 Runt-related transcription factor 1 
Protein Synonyms/Alias
 Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B 
Gene Name
 Runx1 
Gene Synonyms/Alias
 Aml1; Cbfa2 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
24STALSPGKMSEALPLacetylation[1]
43GGAALASKLRSGDRSacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Inhibits KAT6B- dependent transcriptional activation (By similarity). 
Sequence Annotation
 DOMAIN 50 178 Runt.
 REGION 80 84 Interaction with DNA (By similarity).
 REGION 135 143 Interaction with DNA (By similarity).
 REGION 168 177 Interaction with DNA (By similarity).
 REGION 290 369 Interaction with KAT6A (By similarity).
 REGION 306 398 Interaction with KAT6B (By similarity).
 BINDING 112 112 Chloride 1 (By similarity).
 BINDING 116 116 Chloride 1; via amide nitrogen (By
 BINDING 139 139 Chloride 2 (By similarity).
 BINDING 170 170 Chloride 2; via amide nitrogen (By
 MOD_RES 14 14 Phosphothreonine (By similarity).
 MOD_RES 21 21 Phosphoserine (By similarity).
 MOD_RES 24 24 N6-acetyllysine (By similarity).
 MOD_RES 43 43 N6-acetyllysine (By similarity).
 MOD_RES 249 249 Phosphoserine; by HIPK2 (By similarity).
 MOD_RES 266 266 Phosphoserine (By similarity).
 MOD_RES 267 267 Phosphoserine (By similarity).
 MOD_RES 272 272 Phosphothreonine; by HIPK2 (By
 MOD_RES 275 275 Phosphoserine; by HIPK2 (By similarity).  
Keyword
 Acetylation; Complete proteome; DNA-binding; Methylation; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 450 AA 
Protein Sequence
MRIPVDASTS RRFTPPSTAL SPGKMSEALP LGAPDGGAAL ASKLRSGDRS MVEVLADHPG 60
ELVRTDSPNF LCSVLPTHWR CNKTLPIAFK VVALGDVPDG TLVTVMAGND ENYSAELRNA 120
TAAMKNQVAR FNDLRFVGRS GRGKSFTLTI TVFTNPPQVA TYHRAIKITV DGPREPRRHR 180
QKLDDQTKPG SLSFSERLSE LEQLRRTAMR VSPHHPAPTP NPRASLNHST AFNPQPQSQM 240
QDARQIQPSP PWSYDQSYQY LGSITSSVHP ATPISPGRAS GMTSLSAELS SRLSTAPDLT 300
AFGDPRQFPT LPSISDPRMH YPGAFTYSPP VTSGIGIGMS AMSSTSRYHT YLPPPYPGSS 360
QAQAGPFQTG SPSYHLYYGT SAGSYQFSMV GGERSPPRIL PPCTNASTGA ALLNPSLPSQ 420
SDVVETEGSH SNSPTNMPPA RLEEAVWRPY 450 
Gene Ontology
 GO:0005604; C:basement membrane; IEA:Compara.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0005509; F:calcium ion binding; IEA:Compara.
 GO:0000975; F:regulatory region DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0048266; P:behavioral response to pain; IEA:Compara.
 GO:0007417; P:central nervous system development; IEA:Compara.
 GO:0060216; P:definitive hemopoiesis; IEA:Compara.
 GO:0035162; P:embryonic hemopoiesis; IEA:Compara.
 GO:0031069; P:hair follicle morphogenesis; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0030099; P:myeloid cell differentiation; IEA:Compara.
 GO:0002318; P:myeloid progenitor cell differentiation; IEA:Compara.
 GO:0030853; P:negative regulation of granulocyte differentiation; IEA:Compara.
 GO:0048666; P:neuron development; IEA:Compara.
 GO:0045766; P:positive regulation of angiogenesis; ISS:UniProtKB.
 GO:0030854; P:positive regulation of granulocyte differentiation; ISS:UniProtKB.
 GO:2000872; P:positive regulation of progesterone secretion; IMP:RGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0071336; P:regulation of hair follicle cell proliferation; IEA:Compara.
 GO:0009966; P:regulation of signal transduction; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; TAS:RGD.
 GO:0001501; P:skeletal system development; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000040; AML1_Runt.
 IPR008967; p53-like_TF_DNA-bd.
 IPR012346; p53/RUNT-type_TF_DNA-bd.
 IPR013524; Runt_dom.
 IPR027384; Runx_central_dom.
 IPR013711; RunxI_C_dom.
 IPR016554; TF_Runt-rel_RUNX. 
Pfam
 PF00853; Runt
 PF08504; RunxI 
SMART
  
PROSITE
 PS51062; RUNT 
PRINTS
 PR00967; ONCOGENEAML1.