CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007212
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GTPase-activating protein BEM2/IPL2 
Protein Synonyms/Alias
 Bud emergence protein 2 
Gene Name
 BEM2 
Gene Synonyms/Alias
 IPL2; SUP9; YER155C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
27SSTSTSHKTTTASTAubiquitination[1]
285GNDGSSLKNDDSSSTubiquitination[2]
921RSSARLSKSLSISEPubiquitination[2]
1042NPATPNQKMRDSLDTubiquitination[2]
1577EKLSDPTKGTQNLRSubiquitination[2]
1706VYRKKTKKVSESEAIubiquitination[2]
2166KNPNDYFKQ******ubiquitination[2]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 GTPase-activating protein (GAP) for RHO1 and RHO2. Involved in the control of cellular morphogenesis. Required for proper bud site selection and bud emergence. 
Sequence Annotation
 DOMAIN 592 859 Ras-GEF.
 DOMAIN 1846 1948 PH.
 DOMAIN 1967 2165 Rho-GAP.
 MOD_RES 129 129 Phosphoserine.
 MOD_RES 283 283 Phosphoserine.
 MOD_RES 1012 1012 Phosphoserine.
 MOD_RES 1016 1016 Phosphoserine.
 MOD_RES 1038 1038 Phosphothreonine.
 MOD_RES 1046 1046 Phosphoserine.
 MOD_RES 1054 1054 Phosphoserine.
 MOD_RES 1128 1128 Phosphoserine.  
Keyword
 Complete proteome; GTPase activation; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2167 AA 
Protein Sequence
MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST SGASPYMHSH 60
HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN LGMYHSDTNT RSSRSIASTL 120
KDDSPSVCSE DEISNSSSQK SNAQDETPIA YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT 180
TLEKSLNTSG ISNSSGTNNN NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT 240
VATNYNSSMT APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND 300
VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP ASNSSIANEN 360
HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV YPHPDLRQDS DGKIISGTIE 420
SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV INLLLMFPLL DHFIKFLKVF NQFGLSFTKN 480
KSRLTNNSTQ FYNISPAVDD SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH 540
NDEISNNLKI KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV 600
HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL LISHLFPTNP 660
EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI ICSIPVLRSS SWKYVPDQSL 720
KTIFKDWVPT IIQLERRQRT SKSTSSVFIL APPNLDDDFT RANVISYFGD LLIHADDLPS 780
DTKFKYLEKK INRTKNAFHK WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS 840
QPPLNIEGIM KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT 900
LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN DNNKVTGVGK 960
IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL VFKSVYDNDG KSRPASMVIE 1020
TPKRFSQHSS MLINNPATPN QKMRDSLDTT GRLSKTLENM DFFNNIGQVS DSLKESIIRV 1080
VLKSSSLEKI FDLLVLTSNI FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV 1140
KLTMDNDIFT ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE 1200
DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH YSDFTDDLCN 1260
NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET ENLLTTLTDL FHGIKSAYQK 1320
QLYRPIGVNR TQKRITDILN SFNTFSFTDL NNIIDDPSFS DDMIRSFQKL HSTNYEDILE 1380
WIYQLDNFIS KKFNLVSKKD WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH 1440
EFEISNLFTW ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS 1500
ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA HAIISPESRN 1560
YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF IEIDDVFSKN CKNLCPCPGW 1620
FISRLLEISQ FVPNMSITNS KLINFDKRRF VNNIISNVLD LIPNEREFPL DIEMSDENPS 1680
KRTTFGRILF NNFEDVNKVY RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK 1740
LEVLLDQEKI LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES 1800
NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS SSNYSLNSIL 1860
SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI MEIINHRNIP AYYYAFKIVM 1920
QNGHEYLIQT ASSSDLTEWI KMIKASKRFS FHSKKYKGKT HNKIFGVPLE DVCERENTLI 1980
PTIVVKLLEE IELRGLDEVG LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA 2040
IAGCFKMYLR ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT 2100
LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV QTILQDFIKN 2160
PNDYFKQ 2167 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0000131; C:incipient cellular bud site; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005100; F:Rho GTPase activator activity; IDA:SGD.
 GO:0030036; P:actin cytoskeleton organization; IGI:SGD.
 GO:0030010; P:establishment of cell polarity; TAS:SGD.
 GO:0031505; P:fungal-type cell wall organization; TAS:SGD.
 GO:0035024; P:negative regulation of Rho protein signal transduction; IMP:SGD.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR001849; Pleckstrin_homology.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00617; RasGEF
 PF00618; RasGEF_N
 PF00620; RhoGAP 
SMART
 SM00233; PH
 SM00147; RasGEF
 SM00229; RasGEFN
 SM00324; RhoGAP 
PROSITE
 PS50003; PH_DOMAIN
 PS00720; RASGEF
 PS50009; RASGEF_CAT
 PS50238; RHOGAP 
PRINTS