| Tag | Content |
|---|
| CPLM ID | CPLM-013553 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ephexin-1 |
Protein Synonyms/Alias | Eph-interacting exchange protein; Neuronal guanine nucleotide exchange factor |
Gene Name | Ngef |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 178 | LYQEYRDKSTLQEIE | ubiquitination | [1] |
|
Reference | [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis. Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J. J Proteome Res. 2012 Sep 7;11(9):4722-32. [ PMID: 22871113] |
Functional Description | Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. |
Sequence Annotation | DOMAIN 264 448 DH.
DOMAIN 480 592 PH.
DOMAIN 603 664 SH3.
REGION 1 264 Regulatory region; modulates activity
MOD_RES 172 172 Phosphotyrosine.
MOD_RES 597 597 Phosphoserine (By similarity). |
Keyword | Cell projection; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Guanine-nucleotide releasing factor; Membrane; Neurogenesis; Phosphoprotein; Reference proteome; SH3 domain. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 701 AA |
Protein Sequence | METKNSEDQG KPQRKSVSSL WKSKHGPAEM RPELPPETAK ETQNEEPRCL IPIQRNSLFN 60
RAMRHKHKAR SMSERRANDQ AGDLPETRKS VNEPLAFNLP QGRLPPWRTP AQRGPGAQEA 120
SESSSTPGNG TTPEECPALT DSPTTLTEAL QMIHPIPADS WRNLIEQIGL LYQEYRDKST 180
LQEIETRRQQ DAEIQGNSDG SQAGEDNAEE EEEEEEEPAS PPERRALPQI CLLSNPHSRF 240
NLWQDLPEIQ SSGVLDILQP EETKLQEAMF ELVTSEASYY KSLNLLVSHF MENERLKKIL 300
HPSEAHILFS NVLDVMAVSE RFLLELEHRM EENIVISDVC DIVYRYAADH FSVYITYVSN 360
QTYQERTYKQ LLQEKTAFRE LIAQLELDPK CKGLPLSSFL ILPFQRITRL KLLVQNILKR 420
VEEGSEREGT ALDAHKELEM VVKACNEGVR KMSRTEQMIS IQKKMEFKIK SVPIISHSRW 480
LLKQGELQQM SGPKTSRTLR TKKLFREIYL FLFNDLLVIC RQIPGDKYQV FDSAPRGLLR 540
VEELEDQGQT LANVFILRLL ENADDREATY MLKASSQSEM KRWMTSLAPN RRTKFVSFTS 600
RLLDCPQVQC VHPYVAQQPD ELTLELADIL NILEKTEDGW IFGERLHDQE RGWFPSSMTE 660
EILNPKIRSQ NLKECFRVHK MEDPQRSQNK DRRKLGSRNR Q 701 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0030426; C:growth cone; IEA:UniProtKB-SubCell. GO:0016020; C:membrane; IEA:UniProtKB-SubCell. GO:0005543; F:phospholipid binding; IEA:InterPro. GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro. GO:0048013; P:ephrin receptor signaling pathway; ISS:UniProtKB. GO:0061002; P:negative regulation of dendritic spine morphogenesis; ISS:UniProtKB. GO:0043087; P:regulation of GTPase activity; ISS:UniProtKB. GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |