CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001864
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Complement C5 
Protein Synonyms/Alias
 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain 
Gene Name
 C5 
Gene Synonyms/Alias
 CPAMD4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
508YLILSKGKIIHFGTRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. 
Sequence Annotation
 DOMAIN 698 732 Anaphylatoxin-like.
 DOMAIN 1532 1676 NTR.
 CARBOHYD 741 741 N-linked (GlcNAc...).
 CARBOHYD 911 911 N-linked (GlcNAc...).
 CARBOHYD 1115 1115 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1630 1630 N-linked (GlcNAc...).
 DISULFID 567 810
 DISULFID 634 669
 DISULFID 698 724
 DISULFID 699 731
 DISULFID 711 732
 DISULFID 856 883
 DISULFID 866 1527
 DISULFID 1101 1159
 DISULFID 1375 1505
 DISULFID 1405 1474
 DISULFID 1520 1525
 DISULFID 1532 1606
 DISULFID 1553 1676
 DISULFID 1654 1657  
Keyword
 3D-structure; Cleavage on pair of basic residues; Complement alternate pathway; Complement pathway; Complete proteome; Cytolysis; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunity; Inflammatory response; Innate immunity; Membrane attack complex; Polymorphism; Reference proteome; Secreted; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1676 AA 
Protein Sequence
MGLLGILCFL IFLGKTWGQE QTYVISAPKI FRVGASENIV IQVYGYTEAF DATISIKSYP 60
DKKFSYSSGH VHLSSENKFQ NSAILTIQPK QLPGGQNPVS YVYLEVVSKH FSKSKRMPIT 120
YDNGFLFIHT DKPVYTPDQS VKVRVYSLND DLKPAKRETV LTFIDPEGSE VDMVEEIDHI 180
GIISFPDFKI PSNPRYGMWT IKAKYKEDFS TTGTAYFEVK EYVLPHFSVS IEPEYNFIGY 240
KNFKNFEITI KARYFYNKVV TEADVYITFG IREDLKDDQK EMMQTAMQNT MLINGIAQVT 300
FDSETAVKEL SYYSLEDLNN KYLYIAVTVI ESTGGFSEEA EIPGIKYVLS PYKLNLVATP 360
LFLKPGIPYP IKVQVKDSLD QLVGGVPVTL NAQTIDVNQE TSDLDPSKSV TRVDDGVASF 420
VLNLPSGVTV LEFNVKTDAP DLPEENQARE GYRAIAYSSL SQSYLYIDWT DNHKALLVGE 480
HLNIIVTPKS PYIDKITHYN YLILSKGKII HFGTREKFSD ASYQSINIPV TQNMVPSSRL 540
LVYYIVTGEQ TAELVSDSVW LNIEEKCGNQ LQVHLSPDAD AYSPGQTVSL NMATGMDSWV 600
ALAAVDSAVY GVQRGAKKPL ERVFQFLEKS DLGCGAGGGL NNANVFHLAG LTFLTNANAD 660
DSQENDEPCK EILRPRRTLQ KKIEEIAAKY KHSVVKKCCY DGACVNNDET CEQRAARISL 720
GPRCIKAFTE CCVVASQLRA NISHKDMQLG RLHMKTLLPV SKPEIRSYFP ESWLWEVHLV 780
PRRKQLQFAL PDSLTTWEIQ GVGISNTGIC VADTVKAKVF KDVFLEMNIP YSVVRGEQIQ 840
LKGTVYNYRT SGMQFCVKMS AVEGICTSES PVIDHQGTKS SKCVRQKVEG SSSHLVTFTV 900
LPLEIGLHNI NFSLETWFGK EILVKTLRVV PEGVKRESYS GVTLDPRGIY GTISRRKEFP 960
YRIPLDLVPK TEIKRILSVK GLLVGEILSA VLSQEGINIL THLPKGSAEA ELMSVVPVFY 1020
VFHYLETGNH WNIFHSDPLI EKQKLKKKLK EGMLSIMSYR NADYSYSVWK GGSASTWLTA 1080
FALRVLGQVN KYVEQNQNSI CNSLLWLVEN YQLDNGSFKE NSQYQPIKLQ GTLPVEAREN 1140
SLYLTAFTVI GIRKAFDICP LVKIDTALIK ADNFLLENTL PAQSTFTLAI SAYALSLGDK 1200
THPQFRSIVS ALKREALVKG NPPIYRFWKD NLQHKDSSVP NTGTARMVET TAYALLTSLN 1260
LKDINYVNPV IKWLSEEQRY GGGFYSTQDT INAIEGLTEY SLLVKQLRLS MDIDVSYKHK 1320
GALHNYKMTD KNFLGRPVEV LLNDDLIVST GFGSGLATVH VTTVVHKTST SEEVCSFYLK 1380
IDTQDIEASH YRGYGNSDYK RIVACASYKP SREESSSGSS HAVMDISLPT GISANEEDLK 1440
ALVEGVDQLF TDYQIKDGHV ILQLNSIPSS DFLCVRFRIF ELFEVGFLSP ATFTVYEYHR 1500
PDKQCTMFYS TSNIKIQKVC EGAACKCVEA DCGQMQEELD LTISAETRKQ TACKPEIAYA 1560
YKVSITSITV ENVFVKYKAT LLDIYKTGEA VAEKDSEITF IKKVTCTNAE LVKGRQYLIM 1620
GKEALQIKYN FSFRYIYPLD SLTWIEYWPR DTTCSSCQAF LANLDEFAED IFLNGC 1676 
Gene Ontology
 GO:0005615; C:extracellular space; TAS:ProtInc.
 GO:0005579; C:membrane attack complex; IEA:UniProtKB-KW.
 GO:0008009; F:chemokine activity; TAS:ProtInc.
 GO:0004866; F:endopeptidase inhibitor activity; IEA:InterPro.
 GO:0000187; P:activation of MAPK activity; TAS:ProtInc.
 GO:0006874; P:cellular calcium ion homeostasis; IEA:Compara.
 GO:0006935; P:chemotaxis; TAS:ProtInc.
 GO:0006956; P:complement activation; TAS:Reactome.
 GO:0006957; P:complement activation, alternative pathway; IEA:UniProtKB-KW.
 GO:0006958; P:complement activation, classical pathway; IEA:UniProtKB-KW.
 GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
 GO:0007186; P:G-protein coupled receptor signaling pathway; TAS:ProtInc.
 GO:0042593; P:glucose homeostasis; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0006954; P:inflammatory response; TAS:ProtInc.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0002523; P:leukocyte migration involved in inflammatory response; IEA:Compara.
 GO:0033602; P:negative regulation of dopamine secretion; IEA:Compara.
 GO:0010951; P:negative regulation of endopeptidase activity; IEA:GOC.
 GO:0010760; P:negative regulation of macrophage chemotaxis; IDA:BHF-UCL.
 GO:0010700; P:negative regulation of norepinephrine secretion; IEA:Compara.
 GO:0045766; P:positive regulation of angiogenesis; IEA:Compara.
 GO:0090197; P:positive regulation of chemokine secretion; IDA:BHF-UCL.
 GO:0050921; P:positive regulation of chemotaxis; IEA:Compara.
 GO:0010575; P:positive regulation vascular endothelial growth factor production; IDA:BHF-UCL. 
Interpro
 IPR009048; A-macroglobulin_rcpt-bd.
 IPR011626; A2M_comp.
 IPR002890; A2M_N.
 IPR011625; A2M_N_2.
 IPR000020; Anaphylatoxin/fibulin.
 IPR018081; Anaphylatoxin_.
 IPR001840; Anaphylatoxn.
 IPR001599; Macroglobln_a2.
 IPR001134; Netrin_domain.
 IPR018933; Netrin_module_non-TIMP.
 IPR008930; Terpenoid_cyclase/PrenylTrfase.
 IPR008993; TIMP-like_OB-fold. 
Pfam
 PF00207; A2M
 PF07678; A2M_comp
 PF01835; A2M_N
 PF07703; A2M_N_2
 PF07677; A2M_recep
 PF01821; ANATO
 PF01759; NTR 
SMART
 SM00104; ANATO
 SM00643; C345C 
PROSITE
 PS00477; ALPHA_2_MACROGLOBULIN
 PS01177; ANAPHYLATOXIN_1
 PS01178; ANAPHYLATOXIN_2
 PS50189; NTR 
PRINTS
 PR00004; ANAPHYLATOXN.