UniProt Accession

 TPIS_ECOLI; P0A858; P04790; Q2M8L5 

Genbank Protein ID

 X00617; L19201; U00096; AP009048 

Genbank Nucleotide ID

 CAA25253.1; AAB03051.1; AAC76901.1; BAE77391.1 

Protein Name

 Triosephosphate isomerase 

Protein Synonyms/Alias

 TIM; Triose-phosphate isomerase 

Gene Name

 tpiA 

Gene Synonyms/Alias

 tpi; b3919; JW3890 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
11	PLVMGNWKLNGSRHM	acetylation	[1]
51	EMYIDMAKREAEGSH	acetylation	[1]
103	SERRTYHKESDELIA	acetylation	[1, 2]
111	ESDELIAKKFAVLKE	acetylation	[1, 2]
138	EAENEAGKTEEVCAR	acetylation	[1, 2]
176	VWAIGTGKSATPAQA	acetylation	[1]
188	AQAQAVHKFIRDHIA	acetylation	[1]
247	DAFAVIVKAAEAAKQ	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

  

Sequence Annotation

 ACT_SITE 95 95 Electrophile (By similarity).
 ACT_SITE 167 167 Proton acceptor (By similarity).
 BINDING 9 9 Substrate (By similarity).
 BINDING 11 11 Substrate (By similarity).  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Gluconeogenesis; Glycolysis; Isomerase; Pentose shunt; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 255 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0004807; F:triose-phosphate isomerase activity; IMP:EcoliWiki.
 GO:0006094; P:gluconeogenesis; IMP:EcoliWiki.
 GO:0006096; P:glycolysis; IEA:HAMAP.
 GO:0006098; P:pentose-phosphate shunt; IEA:HAMAP. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR022896; TrioseP_Isoase_bac/euk.
 IPR000652; Triosephosphate_isomerase.
 IPR020861; Triosephosphate_isomerase_AS. 

Pfam

 PF00121; TIM 

SMART

  

PROSITE

 PS00171; TIM_1
 PS51440; TIM_2 

PRINTS