CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007529
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ammonium transporter MEP2 
Protein Synonyms/Alias
  
Gene Name
 MEP2 
Gene Synonyms/Alias
 AMT2; YNL142W; N1207; N1820 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
482VGNTDAEKNSTPSDAubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth. 
Sequence Annotation
 CARBOHYD 4 4 N-linked (GlcNAc...).  
Keyword
 Ammonia transport; Complete proteome; Glycoprotein; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 499 AA 
Protein Sequence
MSYNFTGTPT GEGTGGNSLT TDLNTQFDLA NMGWIGVASA GVWIMVPGIG LLYSGLSRKK 60
HALSLLWASM MASAVCIFQW FFWGYSLAFS HNTRGNGFIG TLEFFGFRNV LGAPSSVSSL 120
PDILFAVYQG MFAAVTGALM LGGACERARL FPMMVFLFLW MTIVYCPIAC WVWNAEGWLV 180
KLGSLDYAGG LCVHLTSGHG GLVYALILGK RNDPVTRKGM PKYKPHSVTS VVLGTVFLWF 240
GWMFFNGGSA GNATIRAWYS IMSTNLAAAC GGLTWMVIDY FRCGRKWTTV GLCSGIIAGL 300
VGITPAAGFV PIWSAVVIGV VTGAGCNLAV DLKSLLRIDD GLDCYSIHGV GGCIGSVLTG 360
IFAADYVNAT AGSYISPIDG GWINHHYKQV GYQLAGICAA LAWTVTVTSI LLLTMNAIPF 420
LKLRLSADEE ELGTDAAQIG EFTYEESTAY IPEPIRSKTS AQMPPPHENI DDKIVGNTDA 480
EKNSTPSDAS STKNTDHIV 499 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0008519; F:ammonium transmembrane transporter activity; IDA:SGD.
 GO:0019740; P:nitrogen utilization; IMP:SGD.
 GO:0007124; P:pseudohyphal growth; IMP:SGD. 
Interpro
 IPR001905; Ammonium_transpt.
 IPR018047; Ammonium_transpt_CS.
 IPR024041; NH4_transpt_AmtB-like_dom. 
Pfam
 PF00909; Ammonium_transp 
SMART
  
PROSITE
 PS01219; AMMONIUM_TRANSP 
PRINTS