CPLM-020355

Genbank Nucleotide ID

Polymerase delta-interacting protein 3

Protein Synonyms/Alias

46 kDa DNA polymerase delta interaction protein; p46; S6K1 Aly/REF-like target; SKAR

Homo sapiens (Human)

Position	Peptide	Type	References
144	GTVTPALKLTKTIQV	ubiquitination	[1, 2]
147	TPALKLTKTIQVPQQ	ubiquitination	[2]
155	TIQVPQQKAMAPLHP	ubiquitination	[2]
178	VVNNHQAKQNLYDLD	ubiquitination	[3]
197	GIASVPTKQMKFAAS	ubiquitination	[2, 3, 4]
223	SSKLSMSKALPLTKV	ubiquitination	[2]
229	SKALPLTKVVQNDAY	ubiquitination	[1, 2]
248	LPSSIRTKALTNMSR	ubiquitination	[2]
307	FCVCGALKRARLVHP	acetylation	[5]
332	DDAITAYKKYNNRCL	ubiquitination	[4]
372	LSDSPSMKKESELPR	ubiquitination	[2]
404	TILKALFKSSGASVT	ubiquitination	[2]
418	TTQPTEFKIKL****	ubiquitination	[2]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]

Functional Description

Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.

DOMAIN 280 351 RRM.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 5 5 Phosphoserine.
MOD_RES 127 127 Phosphoserine.
MOD_RES 140 140 Phosphothreonine.
MOD_RES 275 275 Phosphoserine.
MOD_RES 383 383 Phosphoserine; by RPS6KB1.
MOD_RES 385 385 Phosphoserine; by RPS6KB1.

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Translation regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; TAS:UniProtKB.
GO:0016607; C:nuclear speck; IDA:UniProtKB.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0045727; P:positive regulation of translation; IMP:UniProtKB.

IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.