CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029969
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Epidermal growth factor receptor substrate 15 
Protein Synonyms/Alias
  
Gene Name
 EPS15 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
413AQLEEQLKEVRKKCAubiquitination[1]
504FKDDPFGKIDPFGGDubiquitination[1]
514PFGGDPFKGSDPFASubiquitination[2, 3]
620VSNVVITKNVFEETSubiquitination[1, 2, 4]
629VFEETSVKSEDEPPAubiquitination[4]
654PCPLPPGKRSINKLDubiquitination[1, 2, 3, 5]
659PGKRSINKLDSPDPFubiquitination[1, 2, 3, 4, 6]
667LDSPDPFKLNDPFQPubiquitination[1, 2, 3, 4, 5, 6]
682FPGNDSPKEKDPEIFubiquitination[1, 4, 6, 7]
684GNDSPKEKDPEIFCDubiquitination[1, 2, 3, 4, 6, 7]
702SATTTTNKEADPSNFubiquitination[6]
726EDMIEWAKRESEREEubiquitination[4, 6, 7]
756ELAIALSKSEISEA*ubiquitination[4, 6, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 762 AA 
Protein Sequence
MAAAAQLSLT QLSSGNPVYE KYYRQVDTGN TGRVLASDAA AFLKKSGLPD LILGKIWDLA 60
DTDGKGILNK QEFFVALRLV ACAQNGLEVS LSSLNLAVPP PRFHDTSSPL LISGTSAAEL 120
PWAVKPEDKA KYDAIFDSLS PVNGFLSGDK VKPVLLNSKL PVDILGRVWE LSDIDHDGML 180
DRDEFAVAMF LVYCALEKEP VPMSLPPALV PPSKRKTWVV SPAEKAKYDE IFLKTDKDMD 240
GFVSGLEVRE IFLKTGLPST LLAHIWSLCD TKDCGKLSKD QFALAFHLIS QKLIKGIDPP 300
HVLTPEMIPP SDRASLQKNI IGSSPVADFS AIKELDTLNN EIVDLQREKN NVEQDLKEKE 360
DTIKQRTSEV QDLQDEVQRE NTNLQKLQAQ KQQVQELLDE LDEQKAQLEE QLKEVRKKCA 420
EEAQLARSSP ELLPSGVTDE NEVTTAVTEK VCSELDNNRH SKEEDPFNVD SSSLTGPVAD 480
TNLDFFQSDP FVGSDPFKDD PFGKIDPFGG DPFKGSDPFA SDCFFRQSTD PFATSSTDPF 540
SAANNSSITS VETLKHNDPF APGGTVVAAS DSATDPFASV FGNESFGGGF ADFSTLSKVN 600
NEDPFRSATS SSVSNVVITK NVFEETSVKS EDEPPALPPK IGTPTRPCPL PPGKRSINKL 660
DSPDPFKLND PFQPFPGNDS PKEKDPEIFC DPFTSATTTT NKEADPSNFA NFSAYPSEED 720
MIEWAKRESE REEEQRLARL NQQEQEDLEL AIALSKSEIS EA 762 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0005509; F:calcium ion binding; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR003903; Ubiquitin-int_motif. 
Pfam
  
SMART
 SM00054; EFh
 SM00027; EH
 SM00726; UIM 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH
 PS50330; UIM 
PRINTS