CPLM-014909

Genbank Nucleotide ID

AN1-type zinc finger protein 6

Protein Synonyms/Alias

Associated with PRK1 protein; Zinc finger A20 domain-containing protein 3

Homo sapiens (Human)

Position	Peptide	Type	References
137	QPSEEQSKSLEKPKQ	ubiquitination	[1, 2]
186	HNCSYNYKADAAEKI	ubiquitination	[3, 4, 5, 6]
195	DAAEKIRKENPVVVG	ubiquitination	[5, 6]
204	NPVVVGEKIQKI***	acetylation	[6, 7]
204	NPVVVGEKIQKI***	ubiquitination	[5, 6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Involved in regulation of TNF-alpha induced NF-kappa-B activation and apoptosis. Involved in modulation of 'Lys-48'- linked polyubiquitination status of TRAF2 and decreases association of TRAF2 with RIPK1. Required for PTS1 target sequence-dependent protein import into peroxisomes and PEX5 stability; may cooperate with PEX6. In vitro involved in PEX5 export from the cytosol to peroxisomes (By similarity).

ZN_FING 8 42 A20-type.
ZN_FING 146 189 AN1-type.

Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Metal-binding; Protein transport; Reference proteome; Transport; Zinc; Zinc-finger.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:InterPro.
GO:0031593; F:polyubiquitin binding; ISS:UniProtKB.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO:0071356; P:cellular response to tumor necrosis factor; IMP:UniProtKB.
GO:0006625; P:protein targeting to peroxisome; ISS:UniProtKB.
GO:0042981; P:regulation of apoptotic process; IMP:UniProtKB.
GO:0043122; P:regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.

IPR002653; Znf_A20.
IPR000058; Znf_AN1.

PF01754; zf-A20
PF01428; zf-AN1

SM00259; ZnF_A20
SM00154; ZnF_AN1

PS51036; ZF_A20
PS51039; ZF_AN1