UniProt Accession

 TBA4A_MOUSE; P68368; P05215; Q3TY31 

Genbank Protein ID

 M13444; AK002427; AK078355; AK158934; BC019959 

Genbank Nucleotide ID

 AAA40502.1; BAB22094.1; BAC37234.1; BAE34732.1; AAH19959.1 

Protein Name

 Tubulin alpha-4A chain 

Protein Synonyms/Alias

 Alpha-tubulin 4; Alpha-tubulin isotype M-alpha-4; Tubulin alpha-4 chain 

Gene Name

 Tuba4a 

Gene Synonyms/Alias

 Tuba4 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
40	DGQMPSDKTIGGGDD	methylation	[1]
60	FCETGAGKHVPRAVF	ubiquitination	[2]
96	PEQLITGKEDAANNY	acetylation	[3]
112	RGHYTIGKEIIDPVL	ubiquitination	[2]
326	YRGDVVPKDVNAAIA	acetylation	[3]
326	YRGDVVPKDVNAAIA	ubiquitination	[2]
336	NAAIAAIKTKRSIQF	ubiquitination	[2]

Reference

 [1] A method for the comprehensive proteomic analysis of membrane proteins.
 Wu CC, MacCoss MJ, Howell KE, Yates JR 3rd.
 Nat Biotechnol. 2003 May;21(5):532-8. [PMID: 12692561]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. 

Sequence Annotation

 NP_BIND 142 148 GTP (Potential).
 MOD_RES 6 6 Phosphoserine (By similarity).
 MOD_RES 48 48 Phosphoserine (By similarity).
 MOD_RES 83 83 Nitrated tyrosine.
 MOD_RES 232 232 Phosphoserine.
 MOD_RES 282 282 Nitrated tyrosine (By similarity).
 MOD_RES 319 319 Phosphotyrosine.
 MOD_RES 432 432 Phosphotyrosine (By similarity).
 MOD_RES 439 439 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Microtubule; Nitration; Nucleotide-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 448 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro.
 GO:0007017; P:microtubule-based process; IEA:InterPro.
 GO:0051258; P:protein polymerization; IEA:InterPro. 

Interpro

 IPR002452; Alpha_tubulin.
 IPR008280; Tub_FtsZ_C.
 IPR000217; Tubulin.
 IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
 IPR023123; Tubulin_C.
 IPR017975; Tubulin_CS.
 IPR003008; Tubulin_FtsZ_GTPase. 

Pfam

 PF00091; Tubulin
 PF03953; Tubulin_C 

SMART

 SM00864; Tubulin
 SM00865; Tubulin_C 

PROSITE

 PS00227; TUBULIN 

PRINTS

 PR01162; ALPHATUBULIN.
 PR01161; TUBULIN.